https://eln.excellgen.com/index.php?action=history&feed=atom&title=E_coli_genome_engineering 2026-10-18T12:34:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=E_coli_genome_engineering&diff=20307&oldid=prev 2019-10-24T13:47:10Z

<p>Created page with &quot;safe harbor: between ybhD ~ybhH TTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTA...&quot;</p> <p><b>New page</b></p><div>safe harbor:<br /> <br /> between ybhD ~ybhH<br /> <br /> TTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTA<br /> <br /> [https://www.atum.bio/eCommerce/cas9/input https://www.atum.bio/eCommerce/cas9/input]<br /> select: Ecoli,&amp;nbsp;[[Category:Protocols]]NGG, Wild-type Cas9, rRNA offset 4 to 10, target sequence, showing 3 , and this one is good:&amp;nbsp;<br /> TGAATTTAATTACTTTTTTA, then copy the top strand :&amp;nbsp;ACTTAAATTAATGAAAAAAT, check it on snapgene viewer</div>

Paul.xu