Paul.xu at 15:14, 31 May 2018

2018-05-31T15:14:02Z

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{| border="1" cellspacing="1" cellpadding="1" style="width:700px;"
|-
| Name
| Clone ID
| Cat #
| Competitor
| Cat #, Size & Price
| style="width: 65px;" | Customer
| style="width: 400px;" | Note
| Status
|-
| Exonuclease I 
| 1570  pT7A3-MBP-(3C)-Exo-1 
| 
| Thermofisher
| EN0582， 20,000 units, $338.00 
| style="width: 65px;" | 王敏，上海， 1000 KUnits, total: $16,900
| Description Exonuclease I (Exo I) degrades single-stranded DNA in a 3'5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1). Applications  Primer removal from PCR mixtures:  prior to PCR product sequencing (2),  for one-tube “megaprimer” PCR mutagenesis (3).  Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.  Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4). Source E.coli cells with a cloned E.coli sbcB gene. 
| 
|-
| FastAP Thermosensitive Alkaline Phosphatase 
| 
| 
| 
| EF0651 ,1,000 units, $73.25 
| style="width: 65px;" | 王敏，上海， 200 KUnits, total: $14650
| Description Thermo Scientific™ FastAP™ Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'- phosphate groups from DNA, RNA and nucleotides. This enzyme also removes phosphate groups from proteins. FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends in 10 min at 37 °C. The enzyme is inactivated in 5 min at 75 °C. Therefore, removal of alkaline phosphatase is not required prior to ligation. Applications  Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.  Simultaneous digestion and dephosphorylation of vector DNA.  PCR product clean-up: nucleotide degradation prior to sequencing of PCR product.  Dephosphorylation of nucleic acid 5’-termini prior to labeling with T4 Polynucleotide Kinase.  Other applications where dephosphorylation of DNA and RNA substrates is necessary.  Protein dephosphorylation. Source E.coli cells with a cloned bacterial AP gene.
| 
|-
| VVTopo 
| 824 pET28b-VVTopo 
| 
| 
| 
| style="width: 65px;" | order # 4988 
| 
| sequenced
|-
| VVN endonuclease 
| 942   
| 
| 
| 
| style="width: 65px;" | Tien Gen 
| for making NGS library 
| 
|-
| T7 endonuclease 1 
| 941 
| 
| 
| 
| style="width: 65px;" | Tien Gen 
| for making NGS library 
| 
|-
| T4 DNA polymerase
| 
| 
| 
| 
| style="width: 65px;" | XIaoli 
| 
| 
|-
| T4 DNA ligase
| 
| 
| 
| 
| style="width: 65px;" | XIaoli 
| 
| 
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| PAK16D ligase 
| 931 
| 
| 
| 
| style="width: 65px;" | for a biotech company in '''Shanghai''' 
| 
| 
|-
|
'''PolyA polymerase, E coli'''

'''940 pET28b-yPolyA-Pol'''

| '''940'''
| EG-150 
| 
| 
| style="width: 65px;" |
王敏

Boston Children's hospital

| style="width: 59px;" | 
| 
|-
| pET28b-Cid1, yeast polyU 
| 927  
| EG-151 
| 
| 
| style="width: 65px;" | Boston Children's hospital
| style="width: 59px;" | yeast polyU polymerase, [http://www.excellgen.net/eln/index.php/927_Cid1 http://www.excellgen.net/eln/index.php/927_Cid1]
| 
|-
| 
| 
| 
| 
| 
| style="width: 65px;" | 
| style="width: 59px;" | 
| 
|-
| PolyA polymerase, Yeast 
| 
| 
| 
| 
| style="width: 65px;" | 王敏 
| style="width: 59px;" | 
| 
|-
| M.sssl
| 1916 
| 
| 
| 
| style="width: 65px;" | Zhao Fangping
| style="width: 59px;" | Thermo EM0821 
| 
|-
| T4 PNK  
| 
| 
| 
| 
| style="width: 65px;" | Zhao Fangping 
| style="width: 59px;" | Invitrogen: VGN2304: T4 PNK kit 
| 
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| Thermosensitive Alkaline Phosphatase. 
| 
| 
| 
| 
| style="width: 65px;" | Zhao Fangping 
| style="width: 59px;" | Invitrogen: EF0654 FastAP Thermosensitive Alkaline Phosphatase. 
| 
|-
| T5 exonuclease pET8XHis-T5Exo
| 1021  
| EG-166 
| 
| 
| style="width: 65px;" | 胡亚东（分子原料线PM） <1833405799@qq.com> 
| style="width: 59px;" | use 2434-pCold-MBP-(Ent-TEV)-T5-Exo, or 1567 pT7A3-T5-Exo for expression 
| 
|}

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Enzymes Requested by Customers - Revision history

Paul.xu at 15:14, 31 May 2018