https://eln.excellgen.com/index.php?action=history&feed=atom&title=Enzymes_for_Antibody_Engineering
Enzymes for Antibody Engineering - Revision history
2026-10-18T03:29:54Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Enzymes_for_Antibody_Engineering&diff=18824&oldid=prev
Paul.xu at 20:49, 25 July 2018
2018-07-25T20:49:59Z
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<p><b>New page</b></p><div>{| border="1" cellspacing="1" cellpadding="1" style="width:700px;"<br />
|-<br />
| Clone ID<br />
| Parent ID<br />
| Name<br />
| Expression or availability<br />
| Status<br />
| Note<br />
|-<br />
| <br/><br />
| <br/><br />
| pAR1219<br/><br />
| <font color="#333333">[https://www.sigmaaldrich.com/catalog/product/SIGMA/T2076 https://www.sigmaaldrich.com/catalog/product/SIGMA/T2076], $187&nbsp;</font><br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 3248&nbsp;<br/><br />
| <br/><br />
| pQE-81L-T7-RNAP-P266L<br/><br />
| <br/><br />
| <br/><br />
| <br />
|-<br />
| 1080<br />
| <br/><br />
| pBad24-RNAPol<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1780<br/><br />
| 1080<br/><br />
| pT7A3-T7-RNAPol-Y639F<br/><br />
| [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797|No]]<br />
| <br/><br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">T7 RNA Polymerase Y639F</span><br/><br />
|-<br />
| 1499<br/><br />
| <br/><br />
| pT7A30-RNaseInhibitor<br />
| [[623_625_637_1494_1499_1734_1735_1740_1747_1750|Yes]]<br/><br />
| <br/><br />
| pig<br />
|-<br />
| 1531<br/><br />
| <br/><br />
| pENTR223-RNH1<br/><br />
| <br/><br />
| <br/><br />
| Human RNase Inhibitor, HsCD00513809<br/><br />
|-<br />
| 1317<br/><br />
| 1316<br />
| pT7A3-RNH1<br/><br />
| [[623_1248_1285_1313_1314_1317_1494_1536_1705_1706_1732|No]]<br />
| <br/><br />
| extra C, won't translate<br/><br />
|-<br />
| 1316<br />
| <br/><br />
| pLX304-RNH1&nbsp;<br/><br />
| <br/><br />
| <br/><br />
| human ribonuclease/angiogenin inhibitor<br/><br />
|-<br />
| 1536&nbsp;<br/><br />
| 1531<br/><br />
| pT7A3-RNH1<br/><br />
| &nbsp;[[623_1248_1285_1313_1314_1317_1494_1536_1705_1706_1732|No]]&nbsp;<br />
| <br/><br />
| <br />
Human RNase Inhibitor. Need to co-express with&nbsp;GroELS, induction at 22 oC and DTT in,&nbsp;0.04%&nbsp;&nbsp;arabinose,&nbsp;<br />
<br />
see&nbsp;[https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-65 https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-65]<br />
<br />
|-<br />
| 1495<br/><br />
| 501<br/><br />
| pT7A3-MMLV<br />
| [[1495-1496-1497-screening|No]]<br />
| <br/><br />
| <br/><br />
|-<br />
| 501<br />
| <br/><br />
| pET30b-MMLV<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1713<br />
| <br/><br />
| MMLV<br/><br />
| <br/><br />
| <br/><br />
| wt MMLV from Mingjie<br />
|-<br />
| 1714<br/><br />
| 1549<br/><br />
| pEC-iMMLV<br/><br />
| <br/><br />
| <br/><br />
| superscript (E69K,E302R,N454K,D524N)<br/><br />
|-<br />
| 1738<br/><br />
| 1714<br/><br />
| pEC-iMMLV-m<br/><br />
| <br/><br />
| <br/><br />
| E69K,E302R,W313F,L435G,N454K,D524N<br/><br />
|-<br />
| 1549<br/><br />
| 1713<br/><br />
| pEC-MMLV-wt<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1858<br/><br />
| 1713<br/><br />
| pEC-fMMLV<br/><br />
| <br/><br />
| <br/><br />
| D200N, L603W, T330P, L139P and E607K<br/><br />
|-<br />
| 1747<br />
| 1738<br />
| pT7A30-iMMLV<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1868<br/><br />
| 1858<br/><br />
| pT7A30-fMMLV<br/><br />
| [[MKpnI_1715_1868_1483_1900_1654_1901_1464_1902_1500_1903_1904|Y]], [[1500_1903_1519_1919_1868_1873_1859_1860_1870_1884_gfp|2]]<br />
| <br/><br />
| D200N, L603W, T330P, L139P and E607K, thermostable at 62°C, has expression<br/><br />
|-<br />
| 1706&nbsp;<br/><br />
| <br/><br />
| pT7A3-BirA<br />
| [[623_1248_1285_1313_1314_1317_1494_1536_1705_1706_1732|Y]]<br />
| <br/><br />
| Biotin ligase<br/><br />
|-<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1498<br/><br />
| <br/><br />
| pT7A3-TaqDNALigase<br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1035&nbsp;<br/><br />
| <br/><br />
| pET28b-Tth-Ligase-K294R<br/><br />
| <br/><br />
| <br/><br />
| high fiderity<br />
|-<br />
| 1735<br />
| <br/><br />
| pT7A30-Exo-Taq-sso7d<br />
| <br/><br />
| <br/><br />
| Taq-sso7D<br/><br />
|-<br />
| <br/><br />
| <br/><br />
| KOD<br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| Phusion<br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| 1765<br/><br />
| 1619<br />
| pT7A3-T4DNAPol<br />
| [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797|Yes]]<br />
| <br/><br />
| T4 DNA Polymerase<br/><br />
|-<br />
| 1619<br/><br />
| <br/><br />
| pET28b-T4DNAPol<br/><br />
| <br/><br />
| <br/><br />
| T4 DNA Polymerase<br/><br />
|-<br />
| <br/><br />
| <br/><br />
| T4 DNA ligase<br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|}<br />
[[Category:Antibodies]]</div>
Paul.xu