https://eln.excellgen.com/index.php?action=history&feed=atom&title=Error_Removal_by_T7_Enonuclease_IError Removal by T7 Enonuclease I - Revision history2026-10-18T15:06:22ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Error_Removal_by_T7_Enonuclease_I&diff=11840&oldid=prevPaul.xu: Created page with "1. After PCR, purify PCR product using spin column, elute with 20 ul water 2. Add 15 units of T7 endonuclease I, 2 ul of 10 X T7 endonuclease I buffer. 3. Incubate at 3..."2014-05-12T20:15:15Z<p>Created page with "1. After PCR, purify PCR product using spin column, elute with 20 ul water 2. Add 15 units of T7 endonuclease I, 2 ul of 10 X T7 endonuclease I buffer. 3. Incubate at 3..."</p>
<p><b>New page</b></p><div>1. After PCR, purify PCR product using spin column, elute with 20 ul water <br />
<br />
2. Add 15 units of T7 endonuclease I,&nbsp; 2 ul of 10 X T7 endonuclease I buffer. <br />
<br />
3. Incubate at 37 oC for 1 hour to digest mismatch DNA <br />
<br />
<br><br> <br />
<br />
[[Category:Protocols]]</div>Paul.xu