https://eln.excellgen.com/index.php?action=history&feed=atom&title=Ethanol_Precipitation_of_Plasmid_DNA 2026-10-18T03:05:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Ethanol_Precipitation_of_Plasmid_DNA&diff=15151&oldid=prev 2016-07-29T19:55:31Z

<p>Created page with &quot;Reagents Needed:&lt;br/&gt;• 3 M sodium acetate pH 5.2 or 5 M ammonium acetate&lt;br/&gt;• DNA&lt;br/&gt;• 100% ethanol&lt;br/&gt;Protocol&lt;br/&gt;1. Measure the volume of the DNA sample.&lt;br/&gt;2. Ad...&quot;</p> <p><b>New page</b></p><div>Reagents Needed:&lt;br/&gt;• 3 M sodium acetate pH 5.2 or 5 M ammonium acetate&lt;br/&gt;• DNA&lt;br/&gt;• 100% ethanol&lt;br/&gt;Protocol&lt;br/&gt;1. Measure the volume of the DNA sample.&lt;br/&gt;2. Add 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M)&lt;br/&gt;&amp;nbsp;- These amounts assume that the DNA is in TE only; if DNA is in a&lt;br/&gt;&amp;nbsp;solution containing salt, adjust salt accordingly to achieve&lt;br/&gt;&amp;nbsp;the correct final concentration.&lt;br/&gt;3. Mix well.&lt;br/&gt;4. Add 2 to 2.5 volumes of cold 100% ethanol (calculated after salt addition). (or 0.6 vol of isopropanol)&lt;br/&gt;5. Mix well.&lt;br/&gt;6. Place on ice or at -20 degrees C for &gt;20 minutes.&lt;br/&gt;7. Spin a maximum speed in a microfuge 10-15 min.&lt;br/&gt;8. Carefully decant supernatant.&lt;br/&gt;9. Add 1 ml 70% ethanol. Mix. Spin briefly. Carefully decant supernatant.&lt;br/&gt;10. Air dry or briefly vacuum dry pellet.&lt;br/&gt;11. Resuspend pellet in the appropriate volume of TE or water.&amp;nbsp;<br /> [[Category:Protocols]]</div>

Paul.xu