https://eln.excellgen.com/index.php?action=history&feed=atom&title=FLP_Cre_VLP_Activity_assay
FLP Cre VLP Activity assay - Revision history
2026-10-18T02:14:22Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=FLP_Cre_VLP_Activity_assay&diff=11841&oldid=prev
Paul.xu at 20:30, 12 May 2014
2014-05-12T20:30:26Z
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<p><b>New page</b></p><div><br> <br />
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= Making VLP<br> =<br />
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2119 pCMV5-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP <br> <br />
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2138 pCMV5-pene-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP <br> <br />
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2136 pCMV5-tat-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP <br> <br />
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2137 pCMV5-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP <br> <br />
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2140 pCMV5-tat-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP <br> <br />
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2139 pCMV5-pene-CD63-Flpe + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP <br> <br />
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2128 pCMV5-Mfge8-Tat-TagGFP-MFGE8 <br> <br />
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2123 pCMV5-Mfge8-Tat-Cre-MFGE8 + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP <br> <br />
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2122 pCMV5-Mfge8-Tat-FLP-MFGE8 + /- 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP <br> <br />
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= Activity assay<br> =<br />
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add 0.5 ml condition culture medium to 24 well plate, 293T cells transfected with 2013 pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP or 1522 CMV-Brainbow-2.1-R<br> <br />
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3 days after transfection <br />
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[[Image:Cre+flp 3days.jpg]]<br> <br />
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7days after transfection<br> <br />
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[[Image:Cre+flp7days1.jpg]]<br> <br />
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[[Category:VLP]]</div>
Paul.xu