https://eln.excellgen.com/index.php?action=history&feed=atom&title=Flpy Flpy - Revision history 2026-10-18T04:02:20Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Flpy&diff=10746&oldid=prev Paul.xu at 21:08, 6 December 2013 2013-12-06T21:08:23Z <p></p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2013/12/5&lt;br&gt; SHANYI <br /> <br /> [[Image:1853 1860.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;982&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | pET28b-yFLP&lt;br&gt; <br /> | rowspan=&quot;9&quot; | yFLP&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; &lt;br&gt; <br /> | 1853&lt;br&gt; <br /> | <br /> 5 ml,&lt;br&gt; <br /> <br /> T7 EXPRESS&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> | rowspan=&quot;9&quot; | <br /> &lt;br&gt; 0.5 mM IPTG <br /> <br /> 37oC 2h&lt;br&gt; <br /> <br /> | rowspan=&quot;9&quot; | Ni beads &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;9&quot; | &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;8&quot; | pT7A30-yFLP&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;8&quot; | 1860&lt;br&gt; <br /> | <br /> 5 ml,&lt;br&gt; <br /> <br /> BL21DE3pRare2pLysS <br /> <br /> |-<br /> | 2&lt;br&gt; <br /> | <br /> &amp;nbsp;5 ml,&lt;br&gt; Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> <br /> |-<br /> | 3&lt;br&gt; <br /> | <br /> 5 ml,&lt;br&gt;<br /> <br /> Rosetta™(DE3)pLysS&lt;br&gt; <br /> <br /> |-<br /> | 4&lt;br&gt; <br /> | <br /> &amp;nbsp;5 ml,&lt;br&gt; Tuner(DE3)pRare2pLysS&lt;br&gt; <br /> <br /> |-<br /> | 5&lt;br&gt; <br /> | <br /> &amp;nbsp;5 ml,&lt;br&gt; Nico21(DE3)plysH&lt;br&gt; <br /> <br /> |-<br /> | 6&lt;br&gt; <br /> | &amp;nbsp; 5 ml, <br /> BL21(DE3)&lt;br&gt; <br /> <br /> |-<br /> | 7&lt;br&gt; <br /> | &amp;nbsp; 5 ml, <br /> Nico21(DE3)&lt;br&gt; <br /> <br /> |-<br /> | 8&lt;br&gt; <br /> | &amp;nbsp; 5 ml, <br /> Tuner(DE3)&lt;br&gt; <br /> <br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu