https://eln.excellgen.com/index.php?action=history&feed=atom&title=Gel-Solubilization-Buffer 2026-10-18T07:59:37Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Gel-Solubilization-Buffer&diff=1432&oldid=prev 2011-08-03T22:22:52Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Recipe I&#039;&#039;&#039;&lt;br&gt; 60% Guanidine Thiocyanate (5.36M)&lt;br&gt;50mM Tris-HCl pH 7.5&lt;br&gt;0.006% Phenol Red&lt;br&gt; <br /> <br /> Use 3 Volume of buffer to 1 gel volume.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Recipe II&#039;&#039;&#039; <br /> <br /> 60% Guanidine Thiocyanate (5.36M)&lt;br&gt;140mM MES (2-[N-Morpholino] ethanesulfonic acid)&lt;br&gt;0.006% Phenol Red&lt;br&gt; &lt;br&gt;Use 3 Volume of buffer to 1 gel volume. <br /> <br /> Phenol Red is only a pH indicator. It changes color from yellow to red over the pH range 6.8 to 8.4. <br /> <br /> Reference: http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0007750&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Recipe III&#039;&#039;&#039; <br /> <br /> 6M Guanidine Thiocyante&lt;br&gt;50mM Tris-HCl pH 7.5&lt;br&gt;20mM EDTA pH 8.0 <br /> <br /> Use 3 Volume of buffer to 1 gel volume.&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Buffer_Recipe]] [[Category:Protocols]]</div>

Paul.xu