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Glycine HCl Buffer - Revision history
2026-10-18T03:26:30Z
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Paul.xu at 20:37, 3 April 2017
2017-04-03T20:37:23Z
<p></p>
<p><b>New page</b></p><div>0.1 M GLYCINE–HCL; PH 2.2–3.6, PKA = 2.35<br />
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Glycine MW: 75.07<br />
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0.2 M Weigh 7.5&nbsp;g glycine , add DI water to 500 ml<br />
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0.2 M HCl: 8.6 ml of Concentrated HCl (N=<span style="color: rgb(34, 34, 34); font-family: Roboto, arial, sans-serif; font-size: small;">11.65), add DI water to 500 ml.</span><br />
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<br/>0.1 M Glycine-HCl: Add x ml 0.2M HCl to 500 ml 0.2 M Glycine, then add DI water to 1000&nbsp;ml<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| x (ml) &nbsp; &nbsp;<br/><br />
| pH<br/><br />
|-<br />
| 50<br/><br />
| 3.6<br />
|-<br />
| 64<br />
| 3.4<br />
|-<br />
| 82<br />
| 3.2<br />
|-<br />
| 168<br />
| 2.8<br />
|-<br />
| 242<br />
| 2.6<br />
|}<br />
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[[Category:Buffer Recipe]]<br />
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To make 1000 ml 0.2 M Glycine-HCl, pH 2.6: 15 g Glycine, + 5 ml conc HCl, DI water to 1 L.</div>
Paul.xu