Paul.xu: Created page with "To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor.
1. Equilibrate the column with 10 ml buffer
2. Apply the sampl..."

2012-03-05T15:32:46Z

Created page with "To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor. 1. Equilibrate the column with 10 ml buffer 2. Apply the sampl..."

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To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor. 1. Equilibrate the column with  10 ml buffer 2. Apply the sample using a 2–5 ml syringe.

The maximum recommended sample volume is 1.5 ml. Use a flow rate between 1–10 ml/min. Discard the eluted buffer from the column. 

3. Change to buffer and proceed with injection. 

If the sample volume is less than 1.5 ml, add buffer until a total of 1.5 ml buffer is eluted. Discard the eluted buffer.

4. Elute the high molecular weight components with the volumes listed in the following table 

 Collect the eluted buffer 

{| border="1"
|-
| Load ml
| Add ml
| Buffer collect ml
| Yield %
| Salt %
| Dilution factor
|-
| 0.25
| 1.25
| 1
| >95
| 0
| 4
|-
| 0.5
| 1
| 1.5
| >95
| <0.1
| 3
|-
| 1
| 0.5
| 2
| >95
| <0.2
| 2
|-
| 1.5
| 0
| 2
| >95
| <0.2
| 1.3
|}

[[Category:Protocols]]

HiTrap Desalting - Revision history

Paul.xu: Created page with "To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor.1. Equilibrate the column with 10 ml buffer2. Apply the sampl..."

Paul.xu: Created page with "To operate the column with a syringe, connect the syringe to the column with the supplied luer adaptor.
1. Equilibrate the column with 10 ml buffer
2. Apply the sampl..."