Paul.xu at 16:54, 21 January 2019

2019-01-21T16:54:25Z

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== '''Low Yield LB medium''' (g/L): ==

tryptone, 10;  yeast extract, 5.0; NaCl, 10.0.

== '''High Yield Medium''' (g/L): ==

Tryptone, 10.0; yeast extract, '''10 or 15'''; NaCl, 10.0--> 930 ml H2O;

'''or '''25 g LB mix powder + '''10 g''' yeast extract --->930 ml H2O;

Trace metal solution, 1 ml/L;

50 X 2G, 20 ml;

20 X P, 50 ml;

Ampicillin, 100 mg/L,  i.e., 1 ml of 1000X Amp or Carbenicillin;

or Kanamycin, 50 mg/L,  i.e., 1 ml of 1000 X Kan;

Antiform, one tiny drop (don't add more than 5 ul).

'''50 X 2G''' (g/L)

Glucose, 200; Glycerol, 100;

Water to 1 L;

Filter sterilize.

(1X: glycerol, 2.0; glucose, 4 or 5)

'''20 X P''' (g/L)

K2HPO4, 10;

KH2PO4, 125;

(NH4)2HPO4, 40;

MgSO4, 20;

Water to 1L;

Autoclave.

(1 X: K2HPO4, 0.5; KH2PO4, 2.5; (NH4)2HPO4, 2.0; MgSO4, 1.0)

'''Trace metal solution''' (g/L):

FeCl3·6H2O, 0.162;

ZnCl2·4H2O, 0.0144;

CoCl2·6H2O, 0.12;

Na2MoO4·2H2O, 0.012;

CaCl2·2H2O, 0.006;

CuSO4·5H2O, 1.9;

H3BO3, 0.5;

HCl (37%, w/v), 37 ml/L

Filter sterilize 

== TB +GP ==

12 g tryptone

24 g yeast extract,

4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

== 2XYT + GP ==

10 g yeast extract 16 g tryptone (or peptone) 5g NaCl 4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

== LB + GP ==

5g yeast extract 10 g tryptone (or peptone) 10 g NaCl 4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

== For Plasmid Production ==

1 Liter K2HPO4, 12.54 g;  KH2PO4, 2.31 g;  D-Glucose, 4.0g;  NH4Cl, 1.0g;  Yeast extract, 12.0g; NaCl, 5.0g;  MgSO4, 0.24g;
[[Category:Protocols]] [[Category:Buffer Recipe]]

High-Yield-Plasmid-Medium - Revision history

Paul.xu at 16:54, 21 January 2019