https://eln.excellgen.com/index.php?action=history&feed=atom&title=High_Density_Bacteria_Medium 2026-10-18T08:43:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=High_Density_Bacteria_Medium&diff=13848&oldid=prev 2015-01-15T18:56:30Z

<p></p> <p><b>New page</b></p><div>== High Density IPIG induction medium&lt;br&gt; ==<br /> <br /> 50 mM Na2HPO4.7H 2 O, 25 mM KH2PO4, pH 8.0–8.2&lt;br&gt;10 mM NaCl,&lt;br&gt;5 mM MgSO4,&lt;br&gt;0.2 mM CaCl2&lt;br&gt;0.1% NH4Cl&lt;br&gt;1.0% Glucose&lt;br&gt;<br /> <br /> LB<br /> <br /> &lt;br&gt;<br /> <br /> == Hig Density Autoinduce ==<br /> <br /> 50 mM Na2HPO4, 50 mM KH2PO4, pH 8.0–8.2&lt;br&gt;50 mM NH4Cl&lt;br&gt;5 mM Na2SO4&lt;br&gt;2 mM MgSO4&lt;br&gt;0.4% glycerol&lt;br&gt;0.05% glucose&lt;br&gt;0.01% ß-lactose&lt;br&gt;<br /> <br /> LB<br /> <br /> == Recipe 1&lt;br&gt; ==<br /> <br /> 6g Na2HPO4.7H2O&lt;br&gt;3g KH2PO4&lt;br&gt;5g NaCl&lt;br&gt;1g NH4Cl&lt;br&gt;10g tryptone&lt;br&gt;10g peptone&lt;br&gt;40g yeast extract&lt;br&gt;autoclave&lt;br&gt;just before use, add 10ml&lt;br&gt;40% glucose and 1ml 1M MgSO4&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == Terrific Broth&lt;br&gt; ==<br /> <br /> Add the following to 800ml distilled H2O&lt;br&gt; 12g Tryptone&lt;br&gt; 24g Yeast extract&lt;br&gt; 4ml Glycerol&lt;br&gt; Adjust to 900ml with distilled H2O&lt;br&gt; Sterilize by autoclaving&lt;br&gt; Allow to cool to room temperature&lt;br&gt; Adjust volume to 1000ml with 100ml of a filter sterilized solution of 0.17M KH2PO4 and 0.72M K2HPO4&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Buffer_Recipe]]</div>

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