Paul.xu: Created page with "=== '''TREC''' === '''TREC_HDimer_F''' 5'-CACATCCCTTTCAACCATGCTG -3' LENGTH 22 GC CONTENT 50 % MEL TTEMP 56.6 ºC '''TREC_HDimer_R''' AGGGCCCGTGCCAGCTGCAG LENGTH..."

2017-02-20T20:27:02Z

Created page with "=== '''TREC''' === '''TREC_HDimer_F''' 5'-CACATCCCTTTCAACCATGCTG -3' LENGTH 22 GC CONTENT 50 % MEL TTEMP 56.6 ºC '''TREC_HDimer_R''' AGGGCCCGTGCCAGCTGCAG LENGTH..."

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===
'''TREC''' ===

'''TREC_HDimer_F'''

5'-CACATCCCTTTCAACCATGCTG -3'

LENGTH 22

GC CONTENT 50 %

MEL TTEMP 56.6 ºC

'''TREC_HDimer_R'''

AGGGCCCGTGCCAGCTGCAG

LENGTH 20

GC CONTENT 75 %

MELT TEMP 68.2 ºC

Delta G: -3.55 kcal/mole Base Pairs3

5' CACATCCCTTTCAACCATGCTG
|||
3' GACGTCGACCGTGCCCGGGA

=== '''Actin''' ===

'''Actin_HDimer_F:'''

GGGCTTCTTGTCCTTTCCTTCCC

LENGTH 23

GC CONTENT 56.5 %

MELT TEMP 60 ºC

'''Actin_HDimer_R''':

5'-GCTCATTGTAGAAGTACTTCAGGG -3'

LENGTH 24

GC CONTENT 45.8 %

MELT TEMP 55 ºC

Delta G: -6.14 kcal/mole Base Pairs3

5'GGGCTTCTTGTCCTTTCCTTCCC
|||
3' GGGACTTCATGAAGATGTTACTCG

=== '''500 bp''' ===

'''HDimer_F3'''

AGCTCGTGAAACACAAGCCC

LENGTH 20

GC CONTENT 55 %

MELT TEMP 58.2 ºC

Delta G: -6.14 kcal/mole Base Pairs3

5' AGCTCGTGAAACACAAGCCC
|||
3' GGGACTTCATGAAGATGTTACTC

tatagcatatgaatgctgcacagaatccttggaattcctgtcaacaaaggtgatgccacatccctttcaaccatgctgacacctctggtttttgtaaaggtgcccactcctgtgcacggtgatgcataggcacctgcaccccgtgcctaaaccctgcagctggcacgggccctgtctgctcttcattcaccgttctcacgagttgcaataagttcagccctccatgtcacactgtgttttccatcGGATCCTTGCTGTCATCTCTTGTGGGCTGTAATCATCGTCTAGGCTTAAGAGTAATATTGCAAAACCTGTCATGCCCACACAAATCTCTCCCTGGCATTGTTGTCTTTGCAGATGTCAGTGAAAGAGAACCAGCAGCTCcacttgttg agctcgtgaaacacaagcccaaggcaacaaaagagcaactgaaagctgttatggatgatttcgcagcttttgtagagaagtgctgcaaggctgccATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGtaggggagctggctgggtggggcagccccgggagcgggcgggaggcaagggcgctttctctgcacaggagcctcccggtttccggggtgggggctgcgcccgtgGGTACCctcagggcttcttgtcctttccttcccagGGCGTGATGGTGGGCATGGGTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCATCCTCACCCTGAAGTACttctacaatgagc

Hot Start PCR QC - Revision history

Paul.xu: Created page with "=== '''TREC''' === '''TREC_HDimer_F''' 5'-CACATCCCTTTCAACCATGCTG -3' LENGTH 22 GC CONTENT 50 % MEL TTEMP 56.6 ºC '''TREC_HDimer_R''' AGGGCCCGTGCCAGCTGCAG LENGTH..."