https://eln.excellgen.com/index.php?action=history&feed=atom&title=Human_Antibody_Cloning
Human Antibody Cloning - Revision history
2026-10-18T14:09:48Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=Human_Antibody_Cloning&diff=17918&oldid=prev
Paul.xu at 16:42, 12 May 2018
2018-05-12T16:42:20Z
<p></p>
<p><b>New page</b></p><div>== Human Lambda Vaiable ==<br />
<pre>L3 VTSYVLTQPPSVSVAPGQTARITCGGNNI---GSKSVHWYQQKPGQAPVLVVYDDSDRPS 57<br />
L1 --QSALTQPRSVSGSPGQSVTISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVSKRPS 58<br />
L2 --QSVLTQPPSVSAAPGQKVTISCSGSSSNIG-NNYVSWYQQLPGTAPKLLIYDNNKRPS 57<br />
. .**** ***&nbsp;:***.. *:* *.. &nbsp;: * **** ** ** *::** ..***<br />
<br />
L3 GIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDSSSDHPT 100<br />
L1 GVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGSYTFH- 100<br />
L2 GIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSA-- 98<br />
*:*:*****:**.:*:* *:&nbsp;::: ******* &nbsp;: .* <br />
</pre><br />
<br />
== Human Kappa Variable ==<br />
<pre>K1 DIQMTQSPSSLSASVGDRVTITCRASQDIT-NYVNWFQQRPGQAPKVLIYGASILETGVP 59<br />
K4 DIQMTQSPSSLSASVGDRVTITCRASQSIS-SYLNWYQQKPGKAPKLLIYAASSLQSGVP 59<br />
K2 --ELTQAPGTLSLSPGERATFSCRSSHSIRSRRVRWYQHKPGQAPRLVIHGVSNRASGIS 58<br />
K3 EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP 60<br />
&nbsp;:**:*.:** * *:*.*::**:*:.: &nbsp;: *:*::**:**:::*:..* &nbsp;:*: <br />
<br />
K1 SRFSGSGSGTDFTFTISSLQPEDIATYYCQQYDTLPLTFGGGTKVDIKR 108<br />
K4 SRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP------------- 95<br />
K2 DRFSGSGSGTDFTLTITRVEPEDFALYYCQVYGASSYTFGQGTKLERKR 107<br />
K3 DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSP------------- 96<br />
.************:**:&nbsp;::***:* **** &nbsp;: <br />
</pre><br />
<br />
== Human Light Chain Kappa Constant ==<br />
<br />
ACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG<br />
<br />
'''TVAAPSVF'''IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVT<span style="color:#FF0000;">KSFNRGEC</span><br />
<pre><br />
kappa --TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQ 58<br />
L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADGSPVKAGVETTK 57<br />
L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADSSPVKAGVETTT 57<br />
* * * **** * * * *** *** * * * <br />
<br />
kappa DSKDS--TYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 106<br />
L1 PSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 106<br />
L2 PSKSNNKYA-ASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 105<br />
** ** * * * * * *** * * <br />
</pre><br />
<br />
== Human Light Chain Lambda 1 Constant ==<br />
<br />
GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG<span style="color:#FF0000;">AAGACAGTGGCCCCTACAGAATGTTCA</span>TAG<br />
<br />
GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS<br />
<pre>L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSK 60<br />
L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSK 60<br />
***** *:*************************************.**********.***<br />
<br />
L1 QSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 106<br />
L2 -SNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 105<br />
*********************************************<br />
<br />
L1 GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAA 60<br />
L2 GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA 60<br />
************** **** * ******************************** ***<br />
<br />
L1 GCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTG 120<br />
L2 GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTG 120<br />
***************** ******** ** ******************** *********<br />
<br />
L1 GCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAA 180<br />
L2 GCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA 180<br />
*************** ********************************* **********<br />
<br />
L1 CAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAG 240<br />
L2 CAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAG 240<br />
** ***************************** ************** ************<br />
<br />
L1 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300<br />
L2 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300<br />
************************************************************<br />
<br />
L1 GCCCCTACAGAATGTTCATAG 321<br />
L2 GCCCCTACAGAATGTTCATAG 321<br />
*********************<br />
</pre><br />
<br />
== Human Light Chain Lambda 2 Constant ==<br />
<br />
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG<span style="color:#FF0000;">AAGACAGTGGCCCCTACAGAATGTTCA</span>TAG<br />
<br />
GQPKAAPS'''VTLFPPSS'''EELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKT<span style="color:#FF0000;">VAPTECS</span><br />
<br />
== Human Heavy Chain G1 Constant ==<br />
<br />
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA<br />
<br />
A'''STKGPSV'''FPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPS<span style="color:#FF0000;">NTKVDK</span>KVEPKSCDKTHT<br />
<br />
CPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK<br />
<br />
to make Fab:<br />
<br />
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPS<span style="color:#FF0000;">NTKVDK</span>KVEPKSCDKTHT&nbsp;<br />
<br />
GS linker&nbsp; &nbsp; aviTag<br />
<br />
GGSGSAG GLNDIFEAQKIEWHE-<br />
<br />
== Human Heavy Chain G2 Constant ==<br />
<br />
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGTCGAGTGCCCACCGTGCCCAGCACCACCTGTGGCAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA<br />
<br />
ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK<br />
<br />
== Human Heavy Chain G3 Constant ==<br />
<br />
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCAGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCTCAAAACCCCACTTGGTGACACAACTCACACATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCAAGGTGCCCAGCACCTGAACTCCTGGGAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGATACCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAGCGGGCAGCCGGAGAACAACTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACATCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCGCTTCACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTCCTAG<br />
<br />
A'''STKGPSV'''FPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSPGK<br />
<br />
== Human Heavy Chain G4 Constant ==<br />
<br />
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGGGGGGACCATCAGTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGATGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAGCCACAGGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAGGCTCACCGTGGACAAGAGCAGGTGGCAGGAGGGGAATGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACACAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA<br />
<br />
ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSPGK<br />
<br />
== Human IgM Constant ==<br />
<br />
G'''SASAPTL'''FPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGN'''<span style="color:#FF0000;">KEKNVPLP</span>'''VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY<br />
<br />
GGGAGTGCATCCGCCCCAACCCTTTTCCCCCTCGTCTCCTGTGAGAATTCCCCGTCGGATACGAGCAGCGTGGCCGTTGGCTGCCTCGCACAGGACTTCCTTCCCGACTCCATCACTTTCTCCTGGAAATACAAGAACAACTCTGACATCAGCAGCACCCGGGGCTTCCCATCAGTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAACACGTGGTGTGCAAAGTCCAGCACCCCAACGGCAAC<span style="color:#FF0000;">AAAGAAAAGAACGTGCCTCTTCCA</span>GTGATTGCTGAGCTGCCTCCCAAAGTGAGCGTCTTCGTCCCACCCCGCGACGGCTTCTTCGGCAACCCCCGCAAGTCCAAGCTCATCTGCCAGGCCACGGGTTTCAGTCCCCGGCAGATTCAGGTGTCCTGGCTGCGCGAGGGGAAGCAGGTGGGGTCTGGCGTCACCACGGACCAGGTGCAGGCTGAGGCCAAAGAGTCTGGGCCCACGACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCGGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGCCTGACCTTCCAGCAGAATGCGTCCTCCATGTGTGTCCCCGATCAAGACATAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCTCACCAAGTCCACCAAGTTGACCTGCCTGGTCACAGACCTGACCACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCATCTGCGAGGATGACTGGAATTCCGGGGAGAGGTTCACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGGCTTCTCTCCCGCGGACGTCTTCGTGCAGTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAACACGGGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGA<br />
<br />
HuIgMRev: TGGAAGAGGCACGTTCTTTTCTTT<br />
<br />
== Human IgE Constant ==<br />
<br />
A'''STQSP'''SVFPLTRCCKNIPSNATSVTLGCLATGYFPEPVMVTWDTGSLNGTTMTLPATTLTLSGHYATISLLTVSGAWAKQMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK<br />
<br />
== Alignment of Variable Regions ==<br />
<pre>IgG#4 QVQLQESGPGLVQPSETLSLTCTVSGDSITSNVYYWGWIRQPPGKGLEWIGSIFHSG-IT 59<br />
IgM#1 QVHLQESGPGLVKPSETLSLTCSVSGGSM--NSFYWNWVRQPPGKGLEWIGYIFYSG-IT 57<br />
IgG#2 QVQLVQSGAEVKKPGSSVKVSCKASGDSF--NSLAISWVRQAPGQGLEWMGGIIPKFNRR 58<br />
IgG#3 QMQVVQSGAEVKKPGSSVTVSCKASGGTF--SNYAISWVRQAPGQGLEWMGGIIPLFGTP 58<br />
IgG#1 QVQLVESGGGVVQPGMSHRLSCAASTLIF--DRHALHWVRQAPGAGLEWVAILSYDGTTD 58<br />
IgM#2 EVQLVESGGGLVQPGGSLRLSCSASGFTF--SSYAMHWVRQAPGKGLEYVSAISSNGGST 58<br />
&nbsp;::::&nbsp;:** &nbsp;:&nbsp;:*.&nbsp;: &nbsp;::* .* &nbsp;: . *:** ** ***::.&nbsp;: <br />
<br />
IgG#4 HYTPSLNSRVTISVDTSKNQFSLRLSSATAADTAVYYCARHW---------AGLYFDSWG 110<br />
IgM#1 NYNPSLRSRVTISVDTSKNQFSLKLSSVTTADTALYYCARGG---------SQYIPSYWG 108<br />
IgG#2 DYAQKFQGRVTITADDSASTAYIELSSLTSDDTALYYCARDAE----WAAGSDYFFDYWG 114<br />
IgG#3 TYSQNFQGRVTITADKSTSTAHMELISLRSEDTAVYYCATDRYRQANFDRARVGWFDPWG 118<br />
IgG#1 YYADSVKGRFTVSRDTSKNTVFLQMNGLRPQDTAVYYCARGRALD-DFADYGGYYFDYWG 117<br />
IgM#2 YYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCVKDRVLE-WLS--TGYAFDIWG 115<br />
* ....*.*:: * * . &nbsp;:.: . ***:***. . **<br />
<br />
IgG#4 QGALVAVSS-------- 119<br />
IgM#1 QGVLVTVSSGSASAPTL 125<br />
IgG#2 QGTLVIVSS-------- 123<br />
IgG#3 QGTLVTVSS-------- 127<br />
IgG#1 QGILVTVSS-------- 126<br />
IgM#2 QGTMVTVSSGSAS---- 128<br />
**&nbsp;:* *** <br />
</pre><br />
<br />
== Alignment of Constant Regions ==<br />
<pre>IGHD APTKAPDVFPIISGCR-HPKDNSPVVLACLITGYHPTS-VTVTWYMGTQS--QPQRTFPE 56<br />
IgM GSASAPTLFPLVSCEN-SPSDTSSVAVGCLAQDFLPDS-ITFSWKYKNNSDISSTRGFPS 58<br />
Ig-hA1 ASPTSPKVFPLSLCST-QPDG--NVVIACLVQGFFPQEPLSVTWSESGQG--VTARNFPP 55<br />
Ig-hA2 ASPTSPKVFPLSLDST-PQDG--NVVVACLVQGFFPQEPLSVTWSESGQN--VTARNFPP 55<br />
IGHE ASTQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEP-VMVTWDTGSLN--GTTMTLPA 57<br />
IgG1 ASTKGPSVFPLAPSSKSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55<br />
IgG3 ASTKGPSVFPLAPCSRSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55<br />
IgG2 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55<br />
IgG4 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55<br />
. .*&nbsp;:**: ..:.** .: * &nbsp;: .:* &nbsp;:* <br />
<br />
IGHD I-QRRDSYYMTSSQLSTPLQQWRQ---GEYKCVVQHTASKSKKEIF-------------- 98<br />
IgM V--LRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEK--N--VPLPVIAELPPK 112<br />
Ig-hA1 SQDASGDLYTTSSQLTLPA----------------------------------------- 74<br />
Ig-hA2 SQDASGDLYTTSSQLTLPA----------------------------------------- 74<br />
IGHE TTLTLSGHYATISLLTV-SGAWAK---QMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPT 113<br />
IgG1 V-LQSSGLYSLSSVVTVPSSSLGT---QTYICNVNHKPSNTKVDKKVE------------ 99<br />
IgG3 V-LQSSGLYSLSSVVTVPSSSLGT---QTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTC 111<br />
IgG2 V-LQSSGLYSLSSVVTVPSSNFGT---QTYTCNVDHKPSNTKVDKTVERK---------- 101<br />
IgG4 V-LQSSGLYSLSSVVTVPSSSLGT---KTYTCNVDHKPSNTKVDKRVESK---------- 101<br />
.. * *&nbsp;: <br />
<br />
IGHD -----------------------------------RWPESPKAQASSVPTAQPQAEGSLA 123<br />
IgM VS-VFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKES 171<br />
Ig-hA1 ------------------------------------------------------------ 74<br />
Ig-hA2 ------------------------------------------------------------ 74<br />
IGHE VK-ILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGE-- 170<br />
IgG1 ------------------------------------------------------------ 99<br />
IgG3 PRCPEPKSCDTPPPCPR-----CPE----P-----------K------------------ 133<br />
IgG2 ------------------------------------------------------------ 101<br />
IgG4 ------------------------------------------------------------ 101<br />
<br />
<br />
IGHD KATTAPATTRNT----GRGGEEKKK--EKEKEEQEERETKTPECPSHT------------ 165<br />
IgM GPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLT--FQQNASSMCVPDQ----------- 218<br />
Ig-hA1 ---------------TQCLAGKSVTCHVKHYTNP--SQDVTVPCPVPSTPPTPSPSTPPT 117<br />
Ig-hA2 ---------------TQCPDGKSVTCHVKHYTNS--SQDVTVPCRVPP------------ 105<br />
IGHE ---LASTQSELTLSQKHWLSDRTYTCQVTYQGHT--FEDSTKKCAD-SNP---------- 214<br />
IgG1 -------------------------------PKSCDKTHTCPPCPAPELL---------- 118<br />
IgG3 ------------------SCDTPPPCPRCPEPKSCDTPPPCPRCPAPELL---------- 165<br />
IgG2 ------------------------------------CCVECPPCPAPPV----------- 114<br />
IgG4 ------------------------------------YGPPCPSCPAPEFL---------- 115<br />
<br />
<br />
IGHD -----QPLGVYLLTP-AVQDLWLRDKATFTCFVVGSDLKD--AHLTWEVAGKVPTGGVEE 217<br />
IgM ----DTAIRVFAIPP-SFASIFLTKSTKLTCLVTDLTTYD-SVTISWTRQNGEAVK-THT 271<br />
Ig-hA1 PSPSCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GVTFTWTPSSGKSAV-QGP 174<br />
Ig-hA2 -PPPCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GATFTWTPSSGKSAV-QGP 161<br />
IGHE -----RGVSAYLSRPSPF-DLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVN-HST 267<br />
IgG1 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHN-AKT 172<br />
IgG3 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHN-AKT 219<br />
IgG2 -----AGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHN-AKT 168<br />
IgG4 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHN-AKT 169<br />
* &nbsp;:&nbsp;: .**&nbsp;:.. . .&nbsp;:.* . <br />
<br />
IGHD GLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSL 277<br />
IgM NISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDV 331<br />
Ig-hA1 --PERDLCGCYSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKS--GNTFRPEV 230<br />
Ig-hA2 --PERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKS--GNTFRPEV 217<br />
IGHE RKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGP-RAAPEV 326<br />
IgG1 KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ-PREPQV 231<br />
IgG3 KPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQ-PREPQV 278<br />
IgG2 KPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQ-PREPQV 227<br />
IgG4 KPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQ-PREPQV 228<br />
* . . . * * * . .:<br />
<br />
IGHD NLLASSDPP---EAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPP--PQPRST 332<br />
IgM YLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAP-G 390<br />
Ig-hA1 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 290<br />
Ig-hA2 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 277<br />
IGHE YAFATPEWPGS-RDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKG---S 382<br />
IgG1 YTLPPSRDELT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 285<br />
IgG3 YTLPPSREEMT-KNQVSLTCLVKGFYPSDIAVEWESSGQ--PENNYNTTPPMLDSD---G 332<br />
IgG2 YTLPPSREEMT-KNQVSLTCLVKGFYPSDISVEWESNGQ--PENNYKTTPPMLDSD---G 281<br />
IgG4 YTLPPSQEEMT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 282<br />
&nbsp;: . &nbsp;: * .* *&nbsp;;::&nbsp;;: * . <br />
<br />
IGHD TFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVT----------DH-- 380<br />
IgM RYFAHSILTVSEEEWNTGETYTCVVAHEALPN-RVTERTVDKSTGKPTLYNVSLVMSDTA 449<br />
Ig-hA1 TFAVTSILRVAAEDWKKGDTFSCMVGHEALPL-AFTQKTIDRLAGKPTHVNVSVVMAEVD 349<br />
Ig-hA2 TYAVTSILRVAAEDWKKGETFSCMVGHEALPL-AFTQKTIDRMAGKPTHINVSVVMAEAD 336<br />
IGHE GFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK-------------- 428<br />
IgG1 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 330<br />
IgG3 SFFLYSKLTVDKSRWQQGNIFSCSVMHEALHN-RFTQKSLSLSPGK-------------- 377<br />
IgG2 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 326<br />
IgG4 SFFLYSRLTVDKSRWQEGNVFSCSVMHEALHN-HYTQKSLSLSLGK-------------- 327<br />
&nbsp;: * * * &nbsp;: * . ** ..:::. . <br />
<br />
IGHD GPMK 384<br />
IgM GTCY 453<br />
Ig-hA1 GTCY 353<br />
Ig-hA2 GTCY 340<br />
IGHE ---- 428<br />
IgG1 ---- 330<br />
IgG3 ---- 377<br />
IgG2 ---- 326<br />
IgG4 ---- 327<br />
</pre><br />
[[Category:Antibodies]]</div>
Paul.xu