Paul.xu at 17:45, 31 January 2018

2018-01-31T17:45:04Z

New page

{| border="1" cellspacing="1" cellpadding="1" style="width:800px;"
|-
| #
| Task
| Pay ($) 
| Deliverables
| Note
|-
| 1
| Come to work
| 10
| Work ~8 hours in the lab, filll tips, make gels, medium etc
| 
|-
| 2
| Shipping
| 5
| Enter PO order into database, print labels, prepare package, deliver to mail room or FedEx office
| 
|-
| 3
| Colony PCR Screening
| 5
| see [[PCR_Screening_and_Restriction_Digestion|protocol]]
| for Paul's clonining task
|-
| 4
| Miniprep & Restriction digestion
| 10
| see [[Screnning_colony_by_restriction_digestion|protocol]] 
| for Paul's clonining task 
|-
| 5
| PCR cloning into pMiniT
| 20
|
including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc

clone must be confrimed by sequencing

| 
|-
| 6
| PCR cloning directly into expression vector
| 35
|
including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc

clone must be confrimed by sequencing

| 
|-
| 7
| Subcloning
| 15
|
including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc

clone must be confrimed by  PCR, digestion  or sequencing

| 
|-
| 8
| Cloning gene fragment into pMiniT
| 20
|
including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc

clone must be confrimed by sequencing

| 
|-
| 9
| Gene synthesis and clone into pMiniT
| 30
|
including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc

clone must be confrimed by sequencing

| 
|-
| 10
| Protein Expression screening
| 10
| must be  >5 clones per experiment
| 
|-
| 11
| Protein purification 
| 150
| from at least 8 L culture, purified protein by Ni column and then Q or SP or other columns, concentrated, dialysed. Purity should be >90% by SDS-PAGE. 
| 
|-
| 12
| 3rd column chromatography
| 30
| further purification using one additional column
| 
|-
| 13
| Cell culture
| 5
| change medium, take care of cultured cells
| 
|-
| 14
| Transfection
| 10
| 
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| 15
| Real Time PCR
| 5
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[[Category:Tasks]]

Incentive pay schemes - Revision history

Paul.xu at 17:45, 31 January 2018