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Invitrogen's 2X PCR Mix - Revision history
2026-10-18T08:20:06Z
Revision history for this page on the wiki
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Paul.xu: Created page with "Invitrogen/Life Technology's stable 2X mix<br/>1:<br/>20 mM Tris—HCl,pH 8.4<br/>50 mM KCl<br/>0.1% Tween20 or NP40 <br/>Taq 20 units<br/>3 mM MgCl2<br/>200 uM dNT..."
2016-08-18T20:38:40Z
<p>Created page with "Invitrogen/Life Technology's stable 2X mix<br/>1:<br/>20 mM Tris—HCl,pH 8.4<br/>50 mM KCl<br/>0.1% Tween20 or NP40 <br/>Taq 20 units<br/>3 mM MgCl2<br/>200 uM dNT..."</p>
<p><b>New page</b></p><div>Invitrogen/Life Technology's &nbsp;stable 2X mix<br/>1:<br/>20 mM Tris—HCl,pH 8.4<br/>50 mM KCl<br/>0.1% Tween20 or NP40&nbsp;<br/>Taq 20 units<br/>3 mM MgCl2<br/>200 uM dNTP<br />
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2:<br/>66 mM Tris—S04 (pH 9.1)&nbsp;<br/>19.8 mM (NH4)2S04<br/>2.2 mM MgSO4<br/>22 units/ml Taq DNA Polymerase<br/>0.22 units/m1 DEEPVENT DNA Polymerase<br/>0.11% Tween—20<br/>0.011% NP-40&nbsp;<br />
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'''Stability'''<br />
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Room Termperature: &nbsp;<12 weeks&nbsp;<br/>4 oC: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 11 months<br />
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3:<br/>66 mM Tris—S04 (pH 9.1)<br/>19.8 mM (NH4)2S04<br/>2.2 mM MgSO4<br/>24.42 units/ml Taq DNA Polymerase<br/>0.242 units/m1 DEEPVENT DNA<br/>Polymerase<br/>0.066% Tween—20<br/>0.066% NP-40&nbsp;<br />
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'''<span style="line-height: 20.8px;">Stability</span>'''<br />
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Room Termperature: &nbsp;<12 weeks&nbsp;<br/>4 oC: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; >11 months&nbsp;<br />
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[[Category:Protocols]]</div>
Paul.xu