Paul.xu: Created page with "1. after dissecting spleen, keep tissues in ice, isolate RNA within 10-30min
2. Use cleaned motor pristle (treated with 0.2N NaoH first, then..."

2019-10-14T15:52:34Z

Created page with "1. after dissecting spleen, keep tissues in ice, isolate RNA within 10-30min 2. Use cleaned motor pristle (treated with 0.2N NaoH first, then..."

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1. after dissecting spleen,  keep tissues in ice,   isolate RNA within 10-30min  2. Use cleaned motor pristle (treated with 0.2N NaoH first, then autoclaved, after that treated with DEPC water). 2. Try to do RNA isolation in an isolated clean room (better to use safety cabinet). 3. Use clean gloves and  tips Sometimes RNA degration came from gel setup (gel chamber, casting tray, comb) so these were similarly treated with o.2N NaoH for 4-6 h, then clean it properl
[[Category:Protocols]]

Isolate RNA from Spleens - Revision history

Paul.xu: Created page with "1. after dissecting spleen, keep tissues in ice, isolate RNA within 10-30min 2. Use cleaned motor pristle (treated with 0.2N NaoH first, then..."

Paul.xu: Created page with "1. after dissecting spleen, keep tissues in ice, isolate RNA within 10-30min
2. Use cleaned motor pristle (treated with 0.2N NaoH first, then..."