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Hylei at 18:47, 31 May 2011
2011-05-31T18:47:24Z
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<p><b>New page</b></p><div>Clone # 200 <br />
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Digest pFastHacI with&nbsp; <br />
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HindIII and SalI <br />
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[[Image:C6-E3 with HindIII SalI.JPG|142x197px]]<br />
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<br> <br />
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HindIII PstI Xho1 StrepII tag broken Xho1 <br> <br />
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5' agctt CTG CAG ctc gag TGG AGC CAC CCG CAG TTC GAA AAG TgA a 3' <br> <br />
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3' a GAC GTC gag ctc ACC TCG GTG GGC GTC AAG CTT TTC AcT tag ct 5' <br> <br />
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StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa <br> <br />
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StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa<br> <br />
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anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.GST<br> <br />
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[[Image:C6-E3 with HindIII SalI]]<br> <br />
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digestion with Xba1+ Pst1<br> --[[User:Paul.xu|Paul.xu]] 18:13, 6 May 2011 (EST) <br />
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[[Category:Cloning]]</div>
Hylei