https://eln.excellgen.com/index.php?action=history&feed=atom&title=Lentivirus-packaging-mix-QC Lentivirus-packaging-mix-QC - Revision history 2026-10-18T15:06:10Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Lentivirus-packaging-mix-QC&diff=842&oldid=prev Paul.xu at 22:23, 22 May 2011 2011-05-22T22:23:17Z <p></p> <p><b>New page</b></p><div>1. pGap-Pol: (big one)&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> EcoR1+ Xho1== 1598 bp&lt;br&gt;1 XhoI-EcoRI 6764-5168 10555 &lt;br&gt;2 EcoRI-XhoI 5169-6763&#039;&#039;&#039;1595&#039;&#039;&#039; <br /> <br /> =========&lt;br&gt;EcoR1 + Nco1&lt;br&gt;1 EcoRI-NcoI 5169-10137 4969 &lt;br&gt;2 NcoI-EcoRI 373-5168 4796 &lt;br&gt;3 NcoI-NcoI 10138-372 2385&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 2. pVSVG (small one)&lt;br&gt; <br /> <br /> 1 EcoRI-NcoI 1186-379 3689 &lt;br&gt;2 NcoI-EcoRI 380-1185 &#039;&#039;&#039;806&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Sigma packaging EcoR1-xho1-Nco1.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Sigma packaging EcoR1.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Sigma packaging mix EcoR1 2.JPG]] <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Sigma packaging mix EcoR1.jpg]]<br /> <br /> <br /> <br /> <br /> <br /> PureG: cut out the top band of undigested sigma mix, digest it with EcoR1 <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> --[[User:Paul.xu|Paul.xu]] 15:25, 20 May 2011 (EST) <br /> <br /> [[Image:PVSVG pGagPol QC 5 22 2011.JPG]] <br /> <br /> --[[User:Paul.xu|Paul.xu]] 17:23, 22 May 2011 (EST)<br /> <br /> [[Category:Protocols]]</div> Paul.xu