Paul.xu: Created page with "1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS) 2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4..."

2012-03-12T03:05:17Z

Created page with "1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS) 2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4..."

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1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS)

2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4°C

3. Spin at 4000 g for 1 h

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[[Category:Protocols]]

Lentivirus Concentration poly-L-lysine - Revision history

Paul.xu: Created page with "1. Make poly-L-lysine hydrobromide (MW 27,400, Sigma), 100 mg/ml with phosphate-buffered saline (PBS) 2. Add 0.005% of PLL to lentivirus medium and incubate for 30 min for at 4..."