Paul.xu: Created page with "== Fractogel EMD Heparin (S) column
==
1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD He..."

2012-02-28T17:14:34Z

Created page with "== Fractogel EMD Heparin (S) column == 1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD He..."

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== Fractogel EMD Heparin (S) column ==

 

1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD Heparin (S) column). 

2. Wash column with  10 ml of 150 mM NaCl. 

3. Elute virus with 20 ml of 350 mM NaCl. Virus should be  eluted at 4 ~8 ml. 

 

 

[[Category:Protocols|Fractogel EMD Heparin (S) column]]

Lentivirus Purification Heparin Affinity Chromatography - Revision history

Paul.xu: Created page with "== Fractogel EMD Heparin (S) column == 1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD He..."

Paul.xu: Created page with "== Fractogel EMD Heparin (S) column
==
1. Load nonconcentrated lentivirus supernatants (35 mL) or 5 ml of concentrated virus to a heparin column (1 mL Fractogel EMD He..."