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<p></p> <p><b>New page</b></p><div>== Virus supernatant. ==<br /> <br /> 1. 2 days after transfection, take out 0.5 ml medium and add to 1 well of 293T cells growing on 12 well plate (50% confuluency). <br /> <br /> 2. Add 1000X polybrene so the final concentration is 1X <br /> <br /> 3. Incubate for 2 to 5 days and monitor cells for infection (GFP or RFP). <br /> <br /> 4. Check cells for fluorescence and bacterial contamination.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> == Purified virus ==<br /> <br /> 1. Add 1, 2.5, 5 and 10 ul of purified (concentrated) virus to 1 well of 293T cells growing on 12 well plate (50% confuluency). <br /> <br /> 2. Add 1000X polybrene so the final concentration is 1X <br /> <br /> 3. Incubate for 2 to 5 days and monitor cells for infection (GFP or RFP). <br /> <br /> 4. Check cells for fluorescence and bacterial contamination.&lt;br&gt;<br /> <br /> [[Category:Protocols]]</div>

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