https://eln.excellgen.com/index.php?action=history&feed=atom&title=Lentivirus_pLVX-PEDFR-1 2026-10-18T09:55:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Lentivirus_pLVX-PEDFR-1&diff=294&oldid=prev 2011-02-18T14:25:01Z

<p></p> <p><b>New page</b></p><div>Notebook: ZHEN TAO #100 <br /> <br /> Page: 001950 <br /> <br /> &lt;br&gt;<br /> <br /> Transfection Reagent: PEIMAX 32.4 ul/15 cm plate. Packaging Mix: v:g=1:3 (0.1ug/ul). 108 ul/15 cm plate plasmid: pLVX-PEDFR-1 (1.6 ug/ul); 7.2 ug/15 cm plate.<br /> <br /> Prepare TR: For one 15 cm plate, in 1ml DMEM(+), add 32.4 ul PEIMAX. Prepare DNA: For one 15 cm plate, in 1 ml DMEM(+), add 108 ul packaging mix and 4.5 ul plasmid (7.2 ug). Add 1ml TR to 1 ml DNA and mix well, keep at room temperature for 20 min, then add it to 15 cm plate drop by drop. &lt;br&gt;<br /> <br /> [[Image:Lentivirus pLVX-PEDFR-1 1day after transfection.JPG]] <br /> <br /> 1 day after transfection. --[[User:Ztao|Ztao]] 09:25, 18 February 2011 (EST)<br /> <br /> [[Category:Lentivirus]]</div>

Ztao