Paul.xu: Created page with "== 1X T4 DNA Ligase Reaction Buffer ==
50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM Dithiothreitol
pH 7.5 @ 25°C == 1X T7 DNA Ligase Reaction Buffer ==
66 ..."

2012-09-09T01:27:33Z

Created page with "== 1X T4 DNA Ligase Reaction Buffer == 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM Dithiothreitol pH 7.5 @ 25°C == 1X T7 DNA Ligase Reaction Buffer == 66 ..."

New page

== 1X T4 DNA Ligase Reaction Buffer ==

 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM Dithiothreitol pH 7.5 @ 25°C

== 1X T7 DNA Ligase Reaction Buffer ==

 66 mM Tris-HCl 10 mM MgCl2 1 mM ATP 1 mM DTT 7.5 % PEG 6000 pH 7.6 @ 25°C

[[Category:Buffer_Recipe]]

Ligase Buffers - Revision history

Paul.xu: Created page with "== 1X T4 DNA Ligase Reaction Buffer == 50 mM Tris-HCl10 mM MgCl21 mM ATP10 mM DithiothreitolpH 7.5 @ 25°C == 1X T7 DNA Ligase Reaction Buffer == 66 ..."

Paul.xu: Created page with "== 1X T4 DNA Ligase Reaction Buffer ==
50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM Dithiothreitol
pH 7.5 @ 25°C == 1X T7 DNA Ligase Reaction Buffer ==
66 ..."