Paul.xu: Created page with "US20120283144 12%1,2-propanediol 14-fold 5-10% PEG 6000 Ethylene glycol (12%) 10-fold In addition to 5-10% PEG 6000 5%-15% glycerol and 1-8% 1,2-PrD Were &nbs..."

2017-06-22T16:22:14Z

Created page with "US20120283144 12%1,2-propanediol 14-fold 5-10% PEG 6000 Ethylene glycol (12%) 10-fold In addition to 5-10% PEG 6000 5%-15% glycerol and 1-8% 1,2-PrD Were &nbs..."

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US20120283144

12%1,2-propanediol  14-fold

5-10% PEG 6000 

Ethylene glycol (12%) 10-fold

In addition to

5-10% PEG 6000 5%-15% glycerol and 1-8% 1,2-PrD Were  included in the reaction mixtures together With blunt-ended double-stranded DNA or DNA With a T/A overhang. The

ligated DNA Was transformed into host cells using chemically competent cells as described in Example 1.An improvement of at least 25% in ef?ciency of ligation Was observed in

multiple samples in Which glycerol and 1,2-PrD Were included in addition to PEG 6000 compared With ligation effciencies in the presence of PEG 6000 only as a control.

[[Category:Protocols]]

Ligation Enhancers - Revision history

Paul.xu: Created page with "US20120283144 12%1,2-propanediol 14-fold 5-10% PEG 6000 Ethylene glycol (12%) 10-fold In addition to 5-10% PEG 6000 5%-15% glycerol and 1-8% 1,2-PrD Were &nbs..."