Paul.xu: Created page with "== 2x Fast Buffer == 132 mM Tris (pH 7.6) '''20 '''mM MgCl2 2 mM DTT 2 mM ATP 15% PEG (MW 6000) 5 minutes for sticky-end ligations 15 minutes for blunt-end ligations. ==..."

2021-01-04T16:26:28Z

Created page with "== 2x Fast Buffer == 132 mM Tris (pH 7.6) '''20 '''mM MgCl2 2 mM DTT 2 mM ATP 15% PEG (MW 6000) 5 minutes for sticky-end ligations 15 minutes for blunt-end ligations. ==..."

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== 2x Fast Buffer ==

132 mM Tris (pH 7.6)

'''20 '''mM MgCl2

2 mM DTT

2 mM ATP

15% PEG (MW 6000) 5 minutes for sticky-end ligations 15 minutes for blunt-end ligations.

== NEB 1X T4 DNA Ligase Reaction Buffer  ==

 50 mM Tris-HCl 10 mM MgCl2 10 mM DTT 1 mM ATP pH 7.5 @ 25°C

[[Category:Protocols]]

Ligation buffer - Revision history

Paul.xu: Created page with "== 2x Fast Buffer == 132 mM Tris (pH 7.6) '''20 '''mM MgCl2 2 mM DTT 2 mM ATP 15% PEG (MW 6000) 5 minutes for sticky-end ligations 15 minutes for blunt-end ligations. ==..."