https://eln.excellgen.com/index.php?action=history&feed=atom&title=Ligation_of_DNA_fragment_and_pMiniT2Ligation of DNA fragment and pMiniT2 - Revision history2026-10-18T10:23:14ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Ligation_of_DNA_fragment_and_pMiniT2&diff=17072&oldid=prevCrm at 21:54, 20 February 20182018-02-20T21:54:50Z<p></p>
<p><b>New page</b></p><div>1.Contratc out with company to synthsize DNA fragments.<br />
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2.See the instruction of the related DNA fragments.<br />
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3.Use 10ul to disolve the Samples.<br />
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4.Transfer 1 ul to ligate with pMiniT2 Vector.<br />
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5.Formula:<br />
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a.1 ul diluted DNA fragments+3 ul H<sub>2</sub>O<br />
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b.0.5 ul pMiniT2 vector<br />
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c.4ul Cloning Mix 1(buffer)<br />
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d.1 ul Cloning Mix 2(Ligase)<br />
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6.Ligate at RT for 15 min.<br />
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7.Then do transformation.<br />
[[Category:Protocols]]</div>Crm