https://eln.excellgen.com/index.php?action=history&feed=atom&title=Liposome_preparation 2026-10-18T06:20:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Liposome_preparation&diff=11596&oldid=prev 2014-04-01T00:07:13Z

<p>Created page with &quot;== Liposome by Extrusion == 1. dissolve &lt;br&gt;DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), &lt;br&gt;POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), &lt;br&gt;LPC(1-stearoyl-2-hy...&quot;</p> <p><b>New page</b></p><div>== Liposome by Extrusion ==<br /> <br /> 1. dissolve &lt;br&gt;DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), &lt;br&gt;POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), &lt;br&gt;LPC(1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine)&lt;br&gt;and cholesterol (Avanti Polar Lipids, Alabaster, AL) individually in chloroform.<br /> <br /> 2. mix lipid components at a molar ratio of 4:4:2 DOPC/POPC/Cholesterol. &lt;br&gt;3. Dry lipid solutions under vacuum for 3 h and rehydrate in MES buffer to a concentration of 0.5 mg/mL. <br /> <br /> 4. extrude lipids 10 times through a 50-nm pore size polycarbonate membrane filter (GE HealthcareLifeScience,Pittsburgh,PA) to form vesicles that were ~100 nm in diameter, determined by dynamic light&lt;br&gt;scattering (Malvern Instruments, Worcestershire, UK)&lt;br&gt;<br /> <br /> <br /> <br /> [[Category:Protocols]]</div>

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