Paul.xu: Created page with "Long PCR Buffer
In our hands tricine buffer works well with Tth but not as well with Taq. The pH is probably critical to the efficiency of amplification of long targets (1..."

2018-07-12T19:34:49Z

Created page with "Long PCR Buffer In our hands tricine buffer works well with Tth but not as well with Taq. The pH is probably critical to the efficiency of amplification of long targets (1..."

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Long PCR Buffer In our hands tricine buffer works well with Tth but not as well with Taq. The pH is probably critical to the efficiency of amplification of long targets (1).

1X Long PCR Buffer 85 mM KOAc 25 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 8% glycerol 1% DMSO (1 to 4% works) 1.2 mM Mg(OAc)2 A 5X buffer stock containing 6 mM Mg(OAc)2 (1.2 mM final concentration) and 5% DMSO (1% final concentration) can easily be made using 500 mM Mg(OAc)2, 1 M Tricine and 1 M KOAc stock solutions as follows:

5X Long PCR Buffer 425 mM KOAc 125 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 40% glycerol 5% DMSO 6.0 mM Mg(OAc)2

10 ml 5X buffer 4.25 ml 1M KOAc 1.25 ml 1M Tricine, pH8.7 @ 25 degrees C (with KOH) 4.00 ml glycerol 0.50 ml DMSO 120 ul 500 mM Mg(OAc)2
[[Category:Buffer Recipe]]

Long PCR Buffer - Revision history

Paul.xu: Created page with "Long PCR BufferIn our hands tricine buffer works well with Tth but not as well with Taq. The pH is probably critical to the efficiency of amplification of long targets (1..."

Paul.xu: Created page with "Long PCR Buffer
In our hands tricine buffer works well with Tth but not as well with Taq. The pH is probably critical to the efficiency of amplification of long targets (1..."