https://eln.excellgen.com/index.php?action=history&feed=atom&title=MBP%2CMMLV%2CUDG_Protein_Screening MBP,MMLV,UDG Protein Screening - Revision history 2026-10-18T04:04:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=MBP,MMLV,UDG_Protein_Screening&diff=7306&oldid=prev Paul.xu at 23:38, 3 December 2012 2012-12-03T23:38:28Z <p></p> <p><b>New page</b></p><div>=== protein expression screening&lt;br&gt; ===<br /> <br /> 360: pDB.His.MBP&lt;br&gt;<br /> <br /> 1428: PT7-MBP&lt;br&gt;<br /> <br /> 1429: pT7A3-MBP&lt;br&gt;<br /> <br /> 501: MMLV&lt;br&gt;<br /> <br /> 1034: UDG&lt;br&gt; <br /> <br /> Data:2012-11-2--2012-11-7&lt;br&gt; <br /> <br /> Exprimenter: yingying lu&lt;br&gt; <br /> <br /> 3 ml sample each ,then 0.2mMIPTG induce 37C for 5 hours <br /> <br /> picture1: supernatant <br /> <br /> {| width=&quot;485&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Clone ID <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; 360 <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; 1428 <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; 1429 <br /> | colspan=&quot;2&quot; | &amp;nbsp; &amp;nbsp; 501 <br /> | colspan=&quot;2&quot; | &amp;nbsp; 1034<br /> |-<br /> | Name&lt;br&gt;<br /> | colspan=&quot;2&quot; | pDB.His.MBP&lt;br&gt;<br /> | colspan=&quot;2&quot; | PT7-MBP&lt;br&gt;<br /> | colspan=&quot;2&quot; | pT7A3-MBP&lt;br&gt;<br /> | colspan=&quot;2&quot; | MMLV&lt;br&gt;<br /> | colspan=&quot;2&quot; | UDG&lt;br&gt;<br /> |-<br /> | Lane <br /> | &amp;nbsp;1&lt;br&gt; <br /> | 2 <br /> | 3&lt;br&gt; <br /> | 4 <br /> | 5 <br /> | 6 <br /> | 7 <br /> | 8 <br /> | 9 <br /> | 10<br /> |-<br /> | IPTG mM <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:360+1428+1429+501+1034 protein screening 11-7-2012.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> Picture2: pellet <br /> <br /> {| width=&quot;485&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Clone ID <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; 360 <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; 1428 <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; 1429 <br /> | colspan=&quot;2&quot; | &amp;nbsp; &amp;nbsp; 501 <br /> | colspan=&quot;2&quot; | &amp;nbsp; 1034<br /> |-<br /> | Lane <br /> | &amp;nbsp;1&lt;br&gt; <br /> | 2 <br /> | 3&lt;br&gt; <br /> | 4 <br /> | 5 <br /> | 6 <br /> | 7 <br /> | 8 <br /> | 9 <br /> | 10<br /> |-<br /> | IPTG mM <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2 <br /> | 0 <br /> | 0.2<br /> |}<br /> <br /> [[Image:360+1428+142501+1034 pellet screening 11-7-2012.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> conclusion: 360 and 1429 express MBP protein; 1034 express UDG in pellet; 1428 has no MBP expression; 501 has no MMLV expression <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu