Paul.xu at 19:38, 16 November 2012

2012-11-16T19:38:37Z

New page

=   1341-pDB.His MBP-3C-53Wt,1342-pDB-His MBP-3C-p53(1-363), 1343-pDB-His MBP-3C-p53(1-342), 1420-pDB.His.MBP.3C =

Data:2012-11-14--2012-11-15

Exprimenter: xiuli

4 ml sample each ,then 0.5 mMIPTG induce 37C for overnight

picture1: supernatant

{| width="551" cellspacing="1" cellpadding="1" border="1"
|-
| Clone ID
| colspan="2" |     1341
| colspan="2" |     1342
| colspan="2" |    1343
| colspan="2" |     1344
| colspan="2" |   1420
| 1375 MBP
|-
| Lane
|  1 
| 2
| 3 
| 4
| 5
| 6
| 7
| 8
| 9
| 10
| 11
|-
| IPTG mM
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 
|}

 

[[Image:1341-1420 screening sup.jpg]]

 Picture2: pellet

 

 

{| width="551" cellspacing="1" cellpadding="1" border="1"
|-
| Clone ID
| colspan="2" |     1341
| colspan="2" |     1342
| colspan="2" |    1343
| colspan="2" |     1344
| colspan="2" |   1420
|-
| Lane
|  1 
| 2
| 3 
| 4
| 5
| 6
| 7
| 8
| 9
| 10
|-
| IPTG mM
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
|}

 

 

[[Image:1341-1420screening-pellet.jpg]]

[[Category:Protein_Purification]]

MBP, 53W,3C - Revision history

Paul.xu at 19:38, 16 November 2012