Paul.xu: Created page with "Mid-passage MCF-10A cells, human breast epithelial cells obtained from the Barbara Ann Karmanos Cancer Institute (passage 120), were cultured in DMEM:F12 media (1:1) supplemented..."

2014-02-03T19:04:26Z

Created page with "Mid-passage MCF-10A cells, human breast epithelial cells obtained from the Barbara Ann Karmanos Cancer Institute (passage 120), were cultured in DMEM:F12 media (1:1) supplemented..."

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Mid-passage MCF-10A cells, human breast epithelial cells obtained from the Barbara Ann Karmanos Cancer Institute (passage 120), were cultured in DMEM:F12 media (1:1) supplemented with 15% equine serum, insulin (10 ug/ml), epidermal growth factor (20 ng/ml), cholera toxin (100 ng/ml), and hydrocortisone (0.5 ug/ml). Parental cells were maintained with penicillin (100 units/ ml), streptomycin (100 ug/ml), and fungizone (0.5 ug/ml). Cells were passaged at 1:3 to 1:6 every 3– 4 days.

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[[Category:Cell_Lines]]

MCF-10A - Revision history

Paul.xu: Created page with "Mid-passage MCF-10A cells, human breast epithelial cells obtained from the Barbara Ann Karmanos Cancer Institute (passage 120), were cultured in DMEM:F12 media (1:1) supplemented..."