Crm at 18:08, 3 May 2017

2017-05-03T18:08:21Z

New page

[[File:111-1567 EXO QHP 4 13 2017.jpg|RTENOTITLE]]

[[File:112-1483 T4 DNA Ligase QHP 4 17 2017.jpg|RTENOTITLE]]

run SDS-PAGE gel '''1567 EXO/1483 T4 DNA ligase''' 04/18/2017

{| style="width: 500px" border="1" cellspacing="1" cellpadding="1"
|-
| 
| rowspan="1" colspan="5" | 1567 
| 
| rowspan="1" colspan="6" | 1483 
|-
| Number 
| 1 
| 2 
| 3 
| 4 
| 5 
| 6 
| 7 
| 8 
| 9 
| 10 
| 11 
| 12 
|-
| sample 
| 23 
| 24 
| 26 
| 27 
| 28 
| MW 
| 16 
| 18 
| 19 
| 24 
| 28 
| 32 
|-
| uL 
| 8 
| 9 
| 5 
| 8 
| 13 
| 10 
| 4 
| 0.2 
| 0.2 
| 1 
| 3 
| 5 
|-
| ddH2O 
| 5 
| 5 
| 10 
| 5 
| \ 
| \ 
| 10 
| 15 
| 15 
| 10 
| 10 
| 10 
|}

[[File:Protein Expression SDS-PAGE 111-1567 EXO & 112-1483 T4 DNA Ligase QHP 4 18 2017.jpg|RTENOTITLE]]

[[File:1696 1707 1567 1673 1697 protein 4-5-2013.JPG]]

1567 2013/4/5 lane8 [http://excellgen.net/eln/index.php/1696_1707_1567_1673_1697 http://excellgen.net/eln/index.php/1696_1707_1567_1673_1697]

Dialysis of sample 1567-24.

'''0.20mg/ml 4.8ml, total 0.96mg. Wash#1 0.14mg/ml, 1ml.'''

1315 RNA ligase elute with Buffer B 04/18/2017

{| style="width: 500px" border="1" cellspacing="1" cellpadding="1"
|-
| 0.19 
| 0.16 
| 0.21 
| 0.28 
| 0.50 
| (1~5) 
|-
| 0.80 
| 1.17 
| 1.10 
| 1.37 
| 1.49 
| (6~10) 
|-
| 0.60 
| 0.87 
| 1.11 
| 1.44 
| 1.85 
| (11~15) 
|-
| 2.15 
| 2.61 
| 3.03 
| 3.20 
| 3.06 
| (16~20) 
|-
| 3.09 
| 3.11 
| 3.00 
| 2.64 
| 2.49 
| (21~25) 
|-
| 2.24 
| 1.97 
| 1.95 
| 2.10 
| 1.45 
| (26~30) 
|-
| 1.66 
| 1.19 
| 1.24 
| 1.12 
| 1.07 
| (31~35) 
|-
| 0.99 
| 1.02 
| 0.88 
| 0.81 
| 0.84 
| (36~40) 
|-
| 1.18 
| 0.86 
| 0.78 
| 0.72 
| 0.68 
| (41~45) 
|-
| 0.62 
| 0.61 
| 0.57 
| 0.55 
| 0.56 
| (46~50) 
|-
| 
| 
| 
| 
| 0.57 
| (51~55) 
|-
| 
| 
| 
| 
| 0.45 
| (56~60) 
|-
| 
| 
| 
| 
| 0.29 
| (61~65) 
|-
| 
| 
| 
| 
| 0.24 
| (66~70) 
|}

3~70
[[Category:My ELN]]

MS 4 18 2017 - Revision history

Crm at 18:08, 3 May 2017