https://eln.excellgen.com/index.php?action=history&feed=atom&title=MS_4_5_2017 MS 4 5 2017 - Revision history 2026-10-18T04:05:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=MS_4_5_2017&diff=15827&oldid=prev Crm at 18:04, 3 May 2017 2017-05-03T18:04:08Z <p></p> <p><b>New page</b></p><div>1458 KOD elute with Q HP column 04/05/2017<br /> <br /> {| style=&quot;width: 500px&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | Number&lt;br/&gt;<br /> | A280&lt;br/&gt;<br /> |-<br /> | 16&lt;br/&gt;<br /> | -0.02&lt;br/&gt;<br /> |-<br /> | 17&lt;br/&gt;<br /> | -0.01&lt;br/&gt;<br /> |-<br /> | 18&lt;br/&gt;<br /> | 0.12&lt;br/&gt;<br /> |-<br /> | 19&lt;br/&gt;<br /> | 0.64&lt;br/&gt;<br /> |-<br /> | 20&lt;br/&gt;<br /> | 2.13&lt;br/&gt;<br /> |-<br /> | 21&lt;br/&gt;<br /> | 4.54&lt;br/&gt;<br /> |-<br /> | 22&lt;br/&gt;<br /> | 6.10&lt;br/&gt;<br /> |-<br /> | 23&lt;br/&gt;<br /> | 4.65&lt;br/&gt;<br /> |-<br /> | 24&lt;br/&gt;<br /> | 4.75&lt;br/&gt;<br /> |-<br /> | 25&lt;br/&gt;<br /> | 4.21&lt;br/&gt;<br /> |-<br /> | 26&lt;br/&gt;<br /> | 1.11&lt;br/&gt;<br /> |-<br /> | 27&lt;br/&gt;<br /> | 0.82&lt;br/&gt;<br /> |-<br /> | 28&lt;br/&gt;<br /> | 0.25&lt;br/&gt;<br /> |-<br /> | 29&lt;br/&gt;<br /> | 0.06&lt;br/&gt;<br /> |-<br /> | 30&lt;br/&gt;<br /> | 0.10&lt;br/&gt;<br /> |}<br /> <br /> 18~28<br /> <br /> run SDS-PAGE gel<br /> <br /> {| style=&quot;width: 500px&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | number&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 2&lt;br/&gt;<br /> | 3&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> | 6&lt;br/&gt;<br /> | 7&lt;br/&gt;<br /> | 8&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 11&lt;br/&gt;<br /> | 12&lt;br/&gt;<br /> |-<br /> | sample&lt;br/&gt;<br /> | 18&lt;br/&gt;<br /> | 19&lt;br/&gt;<br /> | 20&lt;br/&gt;<br /> | 21&lt;br/&gt;<br /> | 22&lt;br/&gt;<br /> | MW&lt;br/&gt;<br /> | 23&lt;br/&gt;<br /> | 25&lt;br/&gt;<br /> | 26&lt;br/&gt;<br /> | 27&lt;br/&gt;<br /> | 28&lt;br/&gt;<br /> | flow through&lt;br/&gt;<br /> |-<br /> | uL&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 2&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 2&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 15&lt;br/&gt;<br /> |-<br /> | ddH&lt;sub&gt;2&lt;/sub&gt;O&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | \&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | \&lt;br/&gt;<br /> |}<br /> <br /> [[File:Protein Expression SDS-PAGE 110-1458 KOD QHP 4 5 2017.jpg|RTENOTITLE]]<br /> <br /> [[File:1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654NEW.jpg]]<br /> <br /> 2013/3/29 lane3&amp;nbsp;[http://excellgen.net/eln/index.php/1097_1138_1458_1494_1505_1513_1558_1562_1573_1593_1654 http://excellgen.net/eln/index.php/1097_1138_1458_1494_1505_1513_1558_1562_1573_1593_1654]<br /> <br /> <br /> <br /> <br /> <br /> Combined 22 with 23, 24 with 25, concentrate these two samples with dialysis.<br /> <br /> &#039;&#039;&#039;&lt;span style=&quot;color:#FF0000&quot;&gt;22/23: 7.44mg/ml 5.5ml, total 40.92mg. Wash#1 1.02mg/ml, 1ml. Wash#2 0.80mg/ml, 1ml.&lt;/span&gt;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;&lt;span style=&quot;color:#FF0000&quot;&gt;24/25: 3.24mg/ml 6ml, total 19.44mg.&lt;/span&gt;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;&lt;span style=&quot;color:#000000&quot;&gt;1458 KOD QC&lt;/span&gt;&#039;&#039;&#039;<br /> <br /> {| style=&quot;width: 500px&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | rowspan=&quot;1&quot; colspan=&quot;4&quot; | 22&lt;br/&gt;<br /> | rowspan=&quot;1&quot; colspan=&quot;4&quot; | 23&lt;br/&gt;<br /> | positive control&lt;br/&gt;<br /> | rowspan=&quot;1&quot; colspan=&quot;4&quot; | 24&lt;br/&gt;<br /> | rowspan=&quot;1&quot; colspan=&quot;4&quot; | 25&lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 2&lt;br/&gt;<br /> | 3&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> | 6&lt;br/&gt;<br /> | 7&lt;br/&gt;<br /> | 8&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 11&lt;br/&gt;<br /> | 12&lt;br/&gt;<br /> | 13&lt;br/&gt;<br /> | 14&lt;br/&gt;<br /> | 15&lt;br/&gt;<br /> | 16&lt;br/&gt;<br /> | 17&lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | 1mg&lt;br/&gt;<br /> | 0.1mg&lt;br/&gt;<br /> | 10ug&lt;br/&gt;<br /> | 1ug&lt;br/&gt;<br /> | 1mg&lt;br/&gt;<br /> | 0.1mg&lt;br/&gt;<br /> | 10ug&lt;br/&gt;<br /> | 1ug&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 1mg&lt;br/&gt;<br /> | 0.1mg&lt;br/&gt;<br /> | 10ug&lt;br/&gt;<br /> | 1ug&lt;br/&gt;<br /> | 1mg&lt;br/&gt;<br /> | 0.1mg&lt;br/&gt;<br /> | 10ug&lt;br/&gt;<br /> | 1ug&lt;br/&gt;<br /> |-<br /> | dNTP&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> |-<br /> | F&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> |-<br /> | R&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> |-<br /> | template&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> | 0.1&lt;br/&gt;<br /> |-<br /> | 5x buffer&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> |-<br /> | H2O&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> | 12.4&lt;br/&gt;<br /> |-<br /> | enzyme&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> | 0.5&lt;br/&gt;<br /> |}<br /> <br /> [[File:4 5 17 1458 KOD QC PCR.jpg|RTENOTITLE]]<br /> <br /> &lt;span style=&quot;color:#FF0000&quot;&gt;&#039;&#039;&#039;Conclusion: 1ug/ml is good enough.&#039;&#039;&#039;&lt;/span&gt;<br /> [[Category:My ELN]]</div> Crm