https://eln.excellgen.com/index.php?action=history&feed=atom&title=MS_5_15_2017
MS 5 15 2017 - Revision history
2026-10-18T03:58:47Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=MS_5_15_2017&diff=15909&oldid=prev
Crm at 18:02, 24 May 2017
2017-05-24T18:02:05Z
<p></p>
<p><b>New page</b></p><div>run SDS-PAGE gel '''<span style="color:#FF0000">1518 T3 Ligase/1542 sso7d ToughTaq</span>''' 05/15/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br/><br />
| rowspan="1" colspan="5" | 1518 T3 Ligase 41.6<br/><br />
| <br/><br />
| rowspan="1" colspan="6" | 1542 sso7d Native ToughTaq 72.0<br/><br />
|-<br />
| Number<br/><br />
| 1<br/><br />
| 2<br/><br />
| 3<br/><br />
| 4<br/><br />
| 5<br/><br />
| 6<br/><br />
| 7<br/><br />
| 8<br/><br />
| 9<br/><br />
| 10<br/><br />
| 11<br/><br />
| 12<br/><br />
|-<br />
| sample<br/><br />
| FT1<br/><br />
| <span style="color:#000000">23</span><br/><br />
| 25<br/><br />
| 29<br/><br />
| FT2<br/><br />
| MW<br/><br />
| FT1<br/><br />
| 25<br/><br />
| 26<br/><br />
| 29<br/><br />
| 30<br/><br />
| FT2<br/><br />
|-<br />
| uL<br/><br />
| 5<br/><br />
| 2.8<br/><br />
| 1.2<br/><br />
| 0.4<br/><br />
| 15<br/><br />
| 10<br/><br />
| 5<br/><br />
| 0.6<br/><br />
| 0.5<br/><br />
| 0.5<br/><br />
| 0.4<br/><br />
| 15<br/><br />
|-<br />
| ddH<sub>2</sub>O<br/><br />
| 10<br/><br />
| 10<br/><br />
| 15<br/><br />
| 15<br/><br />
| \<br/><br />
| \<br/><br />
| 10<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| \<br/><br />
|}<br />
<br />
[[File:Protein Expression SDS-PAGE 119-1518 T3 DNA Ligase and 120-1542 Native ToughTaq QHP 5 15 2017.jpg|RTENOTITLE]]&nbsp;<br />
<br />
<br />
<br />
Protein Expression Screening of 483 Tth Polymerase /1464 T7 DNA Ligase / 1946 TdT bovine / 1497 ToughTaq<br />
<br />
{| cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| Number<br/><br />
| 1<br/><br />
| 2<br/><br />
| 3<br/><br />
| 4<br/><br />
| 5<br/><br />
| 6<br/><br />
| 7<br/><br />
| 8<br/><br />
| 9<br/><br />
| 10<br/><br />
| 11<br/><br />
| 12<br/><br />
|-<br />
| sample<br/><br />
| <br />
483<br />
<br />
1<br />
<br />
| <br />
<span style="color:#000000">1464</span><br />
<br />
<span style="color:#000000">1</span><br />
<br />
| <br />
1946<br />
<br />
1<br />
<br />
| <br />
1497<br />
<br />
1<br />
<br />
| MW<br/><br />
| <br />
483<br />
<br />
2<br />
<br />
| <br />
1464<br />
<br />
2<br />
<br />
| <br />
1946<br />
<br />
2<br />
<br />
| <br />
1497<br />
<br />
2<br />
<br />
| <br />
1518<br />
<br />
28<br />
<br />
| <br />
1518<br />
<br />
30<br />
<br />
| <br />
1542<br />
<br />
31<br />
<br />
|-<br />
| uL<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 10<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 1.3<br/><br />
| 0.8<br/><br />
| 0.8<br/><br />
|-<br />
| ddH<sub>2</sub>O<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| \<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
|}<br />
<br />
1: induced with 0.5 mM IPTG<br />
<br />
2: without induce<br />
<br />
[[File:Protein Screening 483 1464 1946 1497 1518(28) 1518(30) 1542(31) 05 15 2017.jpg|RTENOTITLE]]<br />
<br />
Conclusion: 483 1464 1946 1497 need to do from plasmid transformation. 1518(29, 30) and 1542( 29,30,31) can forward to the next step.<br />
[[Category:My ELN]]</div>
Crm