Crm at 17:55, 6 June 2017

2017-06-06T17:55:31Z

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Prepare competent cell (Nico21)

Run SDS-PAGE gel of 121-1715 Pfu and 122-1315 Mth RNA Ligase.

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| 
| rowspan="1" colspan="5" | 1715 Pfu 98.6 
| 
| rowspan="1" colspan="6" | 1315 Mth RNA Ligase 45.3 
|-
| Number 
| 1 
| 2 
| 3 
| 4 
| 5 
| 6 
| 7 
| 8 
| 9 
| 10 
| 11 
| 12 
|-
| sample 
| 14 
| 15 
| 17 
| 18 
| 19 
| MW 
| 25 
| 27 
| 33 
| 35 
| 36 
| 44 
|-
| uL 
| 0.4 
| 1.0 
| 0.9 
| 0.4 
| 0.8 
| 10 
| 0.9 
| 1.0 
| 0.5 
| 0.2 
| 0.2 
| 0.9 
|-
| ddH2O 
| 15 
| 15 
| 15 
| 15 
| 15 
| \ 
| 15 
| 15 
| 15
| 15 
| 15 
| 15 
|}

1~5:1715

6:MW

7~12:1315

[[File:Protein Expression SDS-PAGE 121-1715 Pfu and 122-1315 Mth RNA Ligase QHP 5 19 2017.jpg|RTENOTITLE]]

Conclusion: forward sample 14 and 15 of 1715, and 33~44 of 1315 to the next step.

Transfaction reagent 50ul/tube

QC of 1542 Native ToughTaq.

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| 1 
| 2 
| 3 
| 4 
| 5 
| 6 
| 7 
|-
|
positive

control

| 1mg/ml 
| 0.1mg/ml 
| 10ug/ml 
| 1ug/ml 
| 0.1ug/ml 
|
negative

control

|-
| 0.5ul 
| 0.5ul 
| 0.5ul 
| 0.5ul 
| 0.5ul 
| 0.5ul 
| 0 
|}

20ul/tube:

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| dNTP 
| template 
| F 
| R 
| 10x Buffer 
| H2O 
| Taq 
|-
| 1ul 
| 0.1ul 
| 1ul 
| 1ul 
| 2ul 
| 14.4ul 
| 0.5ul 
|}

[[File:1542 Tough Taq QC 5 19 2017.JPG|RTENOTITLE]]

Conclusion: need to do again with less cycle.

[[Category:My ELN]]

MS 5 19 2017 - Revision history

Crm at 17:55, 6 June 2017