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MS 5 23 2017 - Revision history
2026-10-18T03:58:42Z
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Crm at 21:04, 24 May 2017
2017-05-24T21:04:08Z
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<p><b>New page</b></p><div>Plasmid transformation. 483/1464/1946/1497 transfer into Nico21(DE3) PlysN, culture with LB/K/C media.<br />
<br />
Order shipment of 4865 (phi29 (φ29) DNA Polymerase).<br />
<br />
Sorting <span style="color: rgb(51, 51, 51); font-family: arial, 宋体, sans-serif; font-size: 12px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">refrigerator</span>.<br />
<br />
Order shipment of 4866 (HTN-GFP, Cell-Permeable Green Fluorescent Protein).<br />
<br />
Culture 1613 1782 2196 1498 2295 for expression screening.<br />
<br />
run SDS-PAGE gel '''1811'''<span style="font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">VPol </span>'''/ 1995'''<span style="font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Kfu</span>'''/ 2409'''<span style="font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">iFLP</span>'''/ 2295'''<span style="font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">yPolyA-Pol</span>'''/ 1458'''<span style="font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">KOD / </span>'''1715Pfu / 1315Mth RNA Ligase''' 05/23/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| Number<br/><br />
| 1<br/><br />
| 2<br/><br />
| 3<br/><br />
| 4<br/><br />
| 5<br/><br />
| 6<br/><br />
| 7<br/><br />
| 8<br/><br />
| 9<br/><br />
| 10<br/><br />
| 11<br/><br />
| 12<br/><br />
|-<br />
| sample<br/><br />
| 1811<br/><br />
| <span style="color:#000000">1995</span><br/><br />
| <br />
2409<br />
<br />
1<br />
<br />
| <br />
2409<br />
<br />
2<br />
<br />
| <br />
2295<br />
<br />
1<br />
<br />
| <br />
2295<br />
<br />
2<br />
<br />
| <br />
1458<br />
<br />
1<br />
<br />
| <br />
1458<br />
<br />
2<br />
<br />
| MW<br/><br />
| <br />
1715<br />
<br />
13<br />
<br />
| <br />
1715<br />
<br />
16<br />
<br />
| <br />
1315<br />
<br />
41<br />
<br />
|-<br />
| uL<br/><br />
| 0.2<br/><br />
| 0.4<br/><br />
| 1.8<br/><br />
| 1.8<br/><br />
| 1.5<br/><br />
| 1.2<br/><br />
| 0.2<br/><br />
| 0.4<br/><br />
| 10<br/><br />
| 0.8<br/><br />
| 1.3<br/><br />
| 0.5<br/><br />
|-<br />
| ddH<sub>2</sub>O<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
| \<br/><br />
| 15<br/><br />
| 15<br/><br />
| 15<br/><br />
|}<br />
<br />
[[File:Protein Expression 1811 1995 2409 2295 1458 1715 1315 05 23 2017.jpg]]<br />
<br />
[[Category:My ELN]]</div>
Crm