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	<id>https://eln.excellgen.com/index.php?action=history&amp;feed=atom&amp;title=MS_6_12_2017</id>
	<title>MS 6 12 2017 - Revision history</title>
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	<updated>2026-10-18T03:58:45Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
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		<id>https://eln.excellgen.com/index.php?title=MS_6_12_2017&amp;diff=16060&amp;oldid=prev</id>
		<title>Crm at 22:41, 14 June 2017</title>
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		<updated>2017-06-14T22:41:09Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;Culture 2295 yPolyA 200ml.&lt;br /&gt;
&lt;br /&gt;
Culture 1483 T4 DNA Ligase 8L, induced with IPTG.&lt;br /&gt;
&lt;br /&gt;
Homogenize 1497 ToughTaq, load onto Ni column.&lt;br /&gt;
&lt;br /&gt;
Dilute 1946 TdT bovine and load onto QHP column.&lt;br /&gt;
&lt;br /&gt;
Induce 1518 T3 Ligase&amp;amp;nbsp; 1601 6His-GST-i3C 1765 T4 DNA Polymerase 1942 phi29 with IPTG for small scale screening.&lt;br /&gt;
&lt;br /&gt;
Prepare LB media 20L.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[[Category:My ELN]]&lt;/div&gt;</summary>
		<author><name>Crm</name></author>
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