Crm: Created page with "Culture 2295 yPolyA 8L, induced with IPTG. Spin down 1483 T4 DNA Ligase. Wash Ni column (bind with 1497 ToughTaq) with Ni washing buffer, Tris-HCl, Buffer B, dilute and load..."

2017-06-14T22:57:25Z

Created page with "Culture 2295 yPolyA 8L, induced with IPTG. Spin down 1483 T4 DNA Ligase. Wash Ni column (bind with 1497 ToughTaq) with Ni washing buffer, Tris-HCl, Buffer B, dilute and load..."

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Culture 2295 yPolyA 8L, induced with IPTG.

Spin down 1483 T4 DNA Ligase.

Wash Ni column (bind with 1497 ToughTaq) with Ni washing buffer, Tris-HCl, Buffer B, dilute and load onto QHP column.

Elute 1497 ToughTaq with Buffer B 06/13/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.38 
| -0.09 
| 0.05 
| 0.91 
| 1.59 
| (1~5) 
|-
| 1.20 
| 0.61 
| 0.36 
| 0.26 
| 0.18 
| (6~10) 
|-
| 0.15 
| 0.11 
| 0.08 
| 0.06 
| 0.05 
| (11~15) 
|}

collect 4~11.

Elute QHP column (load with 1946 TdT bovine)

[[File:127-1946 TdT bovine QHP 6 13 2017.jpg]]

Resuspend 1498 Taq DNA Ligase, homogenize, load onto Ni column.

Spin down 1518 T3 Ligase  1601 6His-GST-i3C 1765 T4 DNA Polymerase 1942 phi29, incubate with Ni beads.

[[Category:My ELN]]

MS 6 13 2017 - Revision history

Crm: Created page with "Culture 2295 yPolyA 8L, induced with IPTG. Spin down 1483 T4 DNA Ligase. Wash Ni column (bind with 1497 ToughTaq) with Ni washing buffer, Tris-HCl, Buffer B, dilute and load..."