Crm at 23:17, 15 June 2017

2017-06-15T23:17:15Z

New page

Troubleshoot the low flow speed problem of Ni column. Wash Ni column (bind with 1483 T4 DNA Ligase) with Ni washing buffer.

Elute QHP column (load with 1498 Taq DNA Ligase)

[[File:129-1498 Taq DNA Ligase QHP 6 15 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1497 ToughTaq  7.40/72.2 06/15/2017

{| style="width:500px" cellspacing="1" cellpadding="0" border="1" width="500"
|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
22

|
23

|
25

|
MW

|
26

|
27

|
28

|
29

|
30

|
31

|
33

|
35

|-
|
uL

|
3.5

|
0.7

|
2.7

|
10

|
2.2

|
3.0

|
2.2

|
2.4

|
2.2

|
1.6

|
2.2

|
4.6

|-
|
ddH2O

|
10

|
15

|
10

|
\

|
10

|
10

|
10

|
10

|
10

|
15

|
10

|
10

|}

[[File:Protein Expression SDS-PAGE 128-1497 ToughTaq QHP 6 15 2017.jpg|RTENOTITLE]]

Forward sample 22~24 to the next step.  

Prepare Buffer A.

Run SDS-PAGE gel of 1498 Taq DNA Ligase  6.56 / 79.1 06/15/2017

{| style="width:500px" cellspacing="1" cellpadding="0" border="1" width="500"
|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
20

|
22

|
23

|
24

|
26

|
27

|
MW

|
28

|
29

|
32

|
35

|
39

|-
|
uL

|
4.8

|
0.8

|
0.3

|
0.2

|
0.6

|
0.9

|
10

|
0.8

|
0.7

|
1.4

|
2.7

|
4.2

|-
|
ddH2O

|
10

|
15

|
15

|
15

|
15

|
15

|
\

|
15

|
15

|
15

|
10

|
10

|}

 [[File:Protein Expression SDS-PAGE 129-1498 Taq DNA Ligase QHP 6 15 2017.jpg]]

Forward sample 37~39 to the next step.

Dialysis. 1946 TdT bovine, 3.3mg/ml, 2.0ml, 6.6mg. Store at -80oC, 1-1-2 (Enzyme 2017)
[[Category:My ELN]]

MS 6 15 2017 - Revision history

Crm at 23:17, 15 June 2017