Crm: Created page with "Culture 1708 HTN Cre (8L), induced with IPTG, over night. Spin down 483 Tth Polymerase. Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse. Culture 14..."

2017-06-01T22:00:22Z

Created page with "Culture 1708 HTN Cre (8L), induced with IPTG, over night. Spin down 483 Tth Polymerase. Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse. Culture 14..."

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Culture 1708 HTN Cre (8L), induced with IPTG, over night.

Spin down 483 Tth Polymerase.

Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse.

Culture 1464 T7 DNA ligase (200ml)

Wash Ni column (bind with 1782 Tth ligase) with Ni washing Buffer/ Tris-HCl/ Buffer B,

Elute 1782 Tth ligase with Buffer B 06/01/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.08 
| 0.12 
| 1.07 
| 3.23 
| 5.68 
| (1~5) 
|-
| 3.70 
| 2.49 
| 1.46 
| 0.87 
| 0.59 
| (6~10) 
|-
| 0.43 
| 0.31 
| 0.30 
| 0.19 
| 0.11 
| (11~15) 
|}

load sample 3~12 onto QHP column.

[[Category:My ELN]]

MS 6 1 2017 - Revision history

Crm: Created page with "Culture 1708 HTN Cre (8L), induced with IPTG, over night. Spin down 483 Tth Polymerase. Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse. Culture 14..."