Crm at 21:16, 23 June 2017

2017-06-23T21:16:56Z

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Culture 1697 pCold-GST-(3c)-wt3C 200ml.

Culture 1613 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">pET28-cold-GST-3C 8L, induced with IPTG at 16oC.

Homogenize 1942 phi29, load onto Ni column.

Elute QHP column (load with 1483 T4 DNA Ligase)

[[File:130-1483 T4 DNA Ligase QHP 6 21 2017.jpg]]

Run SDS-PAGE gel of 1483 T4 DNA Ligase  6.63 / 57.3  06/21/2017

{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"
|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|-
|
sample

|
10

|
11

|
12

|
13

|
14

|
15

|
16

|
MW

|-
|
uL

|
8.4

|
0.16

|
0.13

|
0.6

|
1.2

|
2.5

|
7.2

|
10

|-
|
ddH2O

|
5

|
15

|
15

|
15

|
15

|
10

|
5

|
\

|}

[[File:Protein Expression SDS-PAGE 130-1483 T4 DNA Ligase QHP 6 21 2017.jpg]]

forward lane 1~3 to the next step.

Dilute 2295 yPolyA and load onto QHP column.

Elute QHP column (load with 2295 yPolyA)

[[File:131-2295 yPolyA QHP 6 21 2017.jpg]]

Miniprep. 2462 Tn5 transposase.

Plasmid transformation of 818 N-6His, Internal 6His. Flag, C-6His / 819 N-His, GST, Myc, C-His / 820 N-6His, GST, HA, C-6XHis / 1708 HTNCre / 2462 Tn5 transposase
[[Category:My ELN]]

MS 6 21 2017 - Revision history

Crm at 21:16, 23 June 2017