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MS 6 22 2017 - Revision history
2026-10-18T07:06:45Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=MS_6_22_2017&diff=16152&oldid=prev
Crm: Created page with "Culture 1518 T3 ligase 200ml. Culture 1697 pCold-GST-(3c)-wt3C 8L, induced with IPTG at 16<sup>o</sup>C. Spin down and homogenize 1613 <span style="color: rgb(37,..."
2017-06-23T21:25:58Z
<p>Created page with "Culture 1518 T3 ligase 200ml. Culture 1697 pCold-GST-(3c)-wt3C 8L, induced with IPTG at 16<sup>o</sup>C. Spin down and homogenize 1613 <span style="color: rgb(37,..."</p>
<p><b>New page</b></p><div>Culture 1518 T3 ligase 200ml.<br />
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Culture 1697&nbsp;pCold-GST-(3c)-wt3C 8L, induced with IPTG at 16<sup>o</sup>C.<br />
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Spin down and homogenize 1613&nbsp;<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">pET28-cold-GST-3C</span> 8L, load onto Ni column.<br />
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Wash Ni column (load with 1942 phi29) with Ni washing Buffer, Tris-HCl, Buffer B.<br />
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Elute QHP column (load with 1518 T3 ligase)<br />
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[[File:132-1518 T3 Ligase QHP 6 22 2017.jpg]]<br />
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Run SDS-PAGE gel of 1518 T3 ligase&nbsp; 5.62 / 41.6&nbsp; 06/22/2017<br />
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{| width="500" cellspacing="1" cellpadding="0" border="1"<br />
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sample<br />
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MW<br />
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uL<br />
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[[File:Protein Expression SDS-PAGE 132-1518 T3 Ligase QHP 6 22 2017.jpg]]<br />
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forward lane 7~8 to the next step.<br />
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Run SDS-PAGE gel of 2295 yPolyA&nbsp; 7.63 / 66.2&nbsp; 06/22/2017<br />
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[[File:Protein Expression SDS-PAGE 131-2295 yPolyA QHP 6 22 2017.jpg]]<br />
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forward lane 1~3 to the next step.<br />
[[Category:My ELN]]</div>
Crm