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MS 6 27 2017 - Revision history
2026-10-18T03:58:46Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=MS_6_27_2017&diff=16235&oldid=prev
Crm: Created page with "Culture 1995 Kfu 200ml.<br/> Culture 1458 KOD 8L, induced with IPTG. Homogenize 1518 T3 ligase, load onto Ni column. Elute 1697 GST-wt3C with Buffer B 06/27/2017 {| style="..."
2017-06-29T00:26:08Z
<p>Created page with "Culture 1995 Kfu 200ml.<br/> Culture 1458 KOD 8L, induced with IPTG. Homogenize 1518 T3 ligase, load onto Ni column. Elute 1697 GST-wt3C with Buffer B 06/27/2017 {| style="..."</p>
<p><b>New page</b></p><div>Culture 1995 Kfu 200ml.<br/><br />
Culture 1458 KOD 8L, induced with IPTG.<br />
<br />
Homogenize 1518 T3 ligase, load onto Ni column.<br />
<br />
Elute 1697 GST-wt3C with Buffer B 06/27/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| -0.21<br/><br />
| -0.21<br/><br />
| -0.21<br/><br />
| 0.00<br/><br />
| 4.41<br/><br />
| (1~5)<br/><br />
|-<br />
| 3.66<br/><br />
| 1.16<br/><br />
| 0.60<br/><br />
| 0.35<br/><br />
| 0.24<br/><br />
| (6~10)<br/><br />
|-<br />
| 0.19<br/><br />
| 0.15<br/><br />
| 0.13<br/><br />
| 0.06<br/><br />
| 0.03<br/><br />
| (11~15)<br/><br />
|}<br />
<br />
collect 5~11.<br />
<br />
Load 1697 GST-wt3C onto QHP column.<br />
<br />
<br />
<br />
Elute 1942 phi29<br />
<br />
[[File:133-1942 phi29 QHP 6 27 2017.jpg]]<br />
<br />
[[Category:My ELN]]</div>
Crm