https://eln.excellgen.com/index.php?action=history&feed=atom&title=MS_6_28_2017 2026-10-18T10:23:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=MS_6_28_2017&diff=16244&oldid=prev 2017-06-30T18:13:40Z

<p></p> <p><b>New page</b></p><div>Culture 1811 VPol 200ml.&lt;br/&gt;Culture 1995 Kfu 8L, induced with IPTG.<br /> <br /> Homogenize 1458 KOD, load onto Ni column.<br /> <br /> Elute 1518 T3 ligase with Buffer B 06/28/2017<br /> <br /> {| style=&quot;width: 500px&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | -0.22&lt;br/&gt;<br /> | -0.20&lt;br/&gt;<br /> | -0.18&lt;br/&gt;<br /> | 0.09&lt;br/&gt;<br /> | 1.22&lt;br/&gt;<br /> | (1~5)&lt;br/&gt;<br /> |-<br /> | 0.97&lt;br/&gt;<br /> | 0.75&lt;br/&gt;<br /> | 0.55&lt;br/&gt;<br /> | 0.41&lt;br/&gt;<br /> | 0.31&lt;br/&gt;<br /> | (6~10)&lt;br/&gt;<br /> |-<br /> | 0.22&lt;br/&gt;<br /> | 0.15&lt;br/&gt;<br /> | 0.11&lt;br/&gt;<br /> | 0.05&lt;br/&gt;<br /> | 0.03&lt;br/&gt;<br /> | (11~15)&lt;br/&gt;<br /> |}<br /> <br /> collect 5~11.<br /> <br /> Load 1518 T3 ligase onto QHP column.<br /> <br /> <br /> <br /> Elute 1697 GST-wt3C<br /> <br /> [[File:134-1697 GST-wt3C QHP 6 28 2017.jpg|RTENOTITLE]]<br /> <br /> <br /> <br /> <br /> <br /> Run SDS-PAGE gel of 1942 phi 29 5.72/70.6&amp;nbsp;&amp;nbsp; 06/28/2017<br /> <br /> {| style=&quot;width:500px&quot; width=&quot;500&quot; cellspacing=&quot;1&quot; cellpadding=&quot;0&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> Number<br /> <br /> | <br /> 1<br /> <br /> | <br /> 2<br /> <br /> | <br /> 3<br /> <br /> | <br /> 4<br /> <br /> | <br /> 5<br /> <br /> | <br /> 6<br /> <br /> |-<br /> | <br /> sample<br /> <br /> | <br /> 11<br /> <br /> | <br /> 12<br /> <br /> | <br /> 13<br /> <br /> | <br /> 14<br /> <br /> | <br /> 15<br /> <br /> | <br /> MW<br /> <br /> |-<br /> | <br /> uL<br /> <br /> | <br /> 12.5<br /> <br /> | <br /> 4.0<br /> <br /> | <br /> 4.2<br /> <br /> | <br /> 5.3<br /> <br /> | <br /> 8.4<br /> <br /> | <br /> 10<br /> <br /> |-<br /> | <br /> ddH&lt;sub&gt;2&lt;/sub&gt;O<br /> <br /> | <br /> \<br /> <br /> | <br /> 10<br /> <br /> | <br /> 10<br /> <br /> | <br /> 10<br /> <br /> | <br /> 5<br /> <br /> | <br /> \<br /> <br /> |}<br /> <br /> [[File:Protein Expression SDS-PAGE 133-1942 phi29 QHP 6 27 2017.jpg|RTENOTITLE]]<br /> <br /> forward lane 1~2 to the next step. <br /> <br /> <br /> <br /> <br /> <br /> Small scale screening of 2462 Tn5 transposase 8.41 / 83.8&amp;nbsp;&amp;nbsp;&amp;nbsp; 818 N-6His 6.12 / 31.9&amp;nbsp;&amp;nbsp;&amp;nbsp; 819 N-His 5.92 / 31.2&amp;nbsp;&amp;nbsp;&amp;nbsp; 820 N-6His 5.99 / 31.2<br /> <br /> Run SDS-PAGE gel &amp;nbsp; 06/28/2017<br /> <br /> {| style=&quot;width:500px&quot; width=&quot;500&quot; cellspacing=&quot;1&quot; cellpadding=&quot;0&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> Number<br /> <br /> | <br /> 1<br /> <br /> | <br /> 2<br /> <br /> | <br /> 3<br /> <br /> | <br /> 4<br /> <br /> | <br /> 5<br /> <br /> |-<br /> | <br /> sample<br /> <br /> | <br /> MW<br /> <br /> | <br /> 2462<br /> <br /> | <br /> 818<br /> <br /> | <br /> 819<br /> <br /> | <br /> 820<br /> <br /> |-<br /> | <br /> uL<br /> <br /> | <br /> 10<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> |}<br /> <br /> [[File:Protein Screening 2462 818 819 820 06 28 2017.jpg|RTENOTITLE]]<br /> <br /> 2462 818 819 820 can do protein purification.<br /> <br /> <br /> <br /> Run SDS-PAGE gel of 1697 GST-wt3C 8.48/21.4 &amp;nbsp; 06/28/2017<br /> <br /> {| style=&quot;width:500px&quot; width=&quot;500&quot; cellspacing=&quot;1&quot; cellpadding=&quot;0&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> Number<br /> <br /> | <br /> 1<br /> <br /> | <br /> 2<br /> <br /> | <br /> 3<br /> <br /> | <br /> 4<br /> <br /> | <br /> 5<br /> <br /> | <br /> 6<br /> <br /> | 7&lt;br/&gt;<br /> | 8&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 11&lt;br/&gt;<br /> | 12&lt;br/&gt;<br /> |-<br /> | <br /> sample<br /> <br /> | <br /> 18<br /> <br /> | <br /> 19<br /> <br /> | <br /> 20<br /> <br /> | <br /> 22<br /> <br /> | <br /> 23<br /> <br /> | <br /> MW<br /> <br /> | 24&lt;br/&gt;<br /> | 25&lt;br/&gt;<br /> | 26&lt;br/&gt;<br /> | 27&lt;br/&gt;<br /> | 29&lt;br/&gt;<br /> | 31&lt;br/&gt;<br /> |-<br /> | <br /> uL<br /> <br /> | <br /> 1.3<br /> <br /> | <br /> 0.9<br /> <br /> | <br /> 0.9<br /> <br /> | <br /> 0.8<br /> <br /> | <br /> 0.8<br /> <br /> | <br /> 10<br /> <br /> | 1.0&lt;br/&gt;<br /> | 1.4&lt;br/&gt;<br /> | 1.8&lt;br/&gt;<br /> | 2.4&lt;br/&gt;<br /> | 4.6&lt;br/&gt;<br /> | 7.7&lt;br/&gt;<br /> |-<br /> | <br /> ddH&lt;sub&gt;2&lt;/sub&gt;O<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> 15<br /> <br /> | <br /> \<br /> <br /> | 15&lt;br/&gt;<br /> | 15&lt;br/&gt;<br /> | 15&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> |}<br /> <br /> [[File:Protein Expression SDS-PAGE 134-1697 GST-wt3C QHP 6 28 2017.jpg]]<br /> <br /> forward lane 1~2 to the next step. <br /> <br /> [[Category:My ELN]]</div>

Crm