Crm at 18:23, 30 June 2017

2017-06-30T18:23:31Z

New page

Culture 2462 Tn5 transposase 200ml. Culture 1811 vPol 8L, induced with IPTG.

Homogenize 1995 Kfu, load onto Ni column.

Wash Ni column (load with 1458 KOD) with Ni washing Buffer/ Tris-HCl/ Buffer B, store at -80oC.

Elute 1458 KOD with Buffer B 06/29/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.02 
| -0.00 
| -0.01 
| 0.09 
| 0.12 
| (1~5) 
|-
| 4.98 
| 18.72 
| 18.77 
| 11.26 
| 6.98 
| (6~10) 
|-
| 4.54 
| 2.84 
| 1.89 
| 1.31 
| 0.92 
| (11~15) 
|-
| 0.72 
| 0.58 
| 0.51 
| 0.44 
| 0.39 
| (16~20) 
|-
| 0.24 
| 0.16 
| 0.13 
| 0.07 
| 0.04 
| (21~25) 
|}

collect 6~21.

Elute 1518 T3 ligase from QHP column.

[[File:135-1518 T3 ligase QHP 6 29 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1518 T3 ligase 5.62/41.6   06/29/2017

{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"
|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
26

|
28

|
29

|
30

|
31

|
32

|
33

|
34

|
MW

|
35

|
36

|
37

|-
|
uL

|
6.7

|
4.8

|
4.2

|
3.4

|
3.5

|
2.5

|
2.0

|
1.8

|
10

|
1.9

|
2.6

|
3.7

|-
|
ddH2O

|
10

|
10

|
10

|
10

|
10

|
10

|
15

|
15

|
\

|
15

|
10

|
10

|}

 [[File:Protein Expression SDS-PAGE 135-1518 T3 ligase QHP 6 29 2017.jpg|RTENOTITLE]]

forward lane 10~12 to the next step.
[[Category:My ELN]]

MS 6 29 2017 - Revision history

Crm at 18:23, 30 June 2017