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MS 6 30 2017 - Revision history
2026-10-18T05:42:13Z
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Crm at 20:27, 4 July 2017
2017-07-04T20:27:58Z
<p></p>
<p><b>New page</b></p><div>Spin down 1811 vPol and store at -20<sup>o</sup>C.<br />
<br />
Wash Ni column (load with 1995 Kfu) with Ni washing Buffer/ Tris-HCl/ Buffer B, store at -80<sup>o</sup>C.<br />
<br />
Elute 1995 Kfu with Buffer B 06/30/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| -0.21<br/><br />
| -0.19<br/><br />
| -0.09<br/><br />
| 0.02<br/><br />
| 1.26<br/><br />
| (1~5)<br/><br />
|-<br />
| 1.09<br/><br />
| 0.85<br/><br />
| 0.69<br/><br />
| 0.58<br/><br />
| 0.58<br/><br />
| (6~10)<br/><br />
|-<br />
| 0.58<br/><br />
| 0.56<br/><br />
| 0.55<br/><br />
| 0.54<br/><br />
| 0.51<br/><br />
| (11~15)<br/><br />
|-<br />
| 0.45<br/><br />
| 0.40<br/><br />
| 0.40<br/><br />
| 0.36<br/><br />
| 0.33<br/><br />
| (16~20)<br/><br />
|-<br />
| 0.32<br/><br />
| 0.30<br/><br />
| 0.21<br/><br />
| 0.14<br/><br />
| 0.08<br/><br />
| (21~25)<br/><br />
|}<br />
<br />
collect 5~22.<br />
<br />
<br />
<br />
Prepare LB media 20L.<br />
<br />
Dialysis of 1942 phi29, 1697 GST-wt3C, 1518 T3 ligase.<br />
<br />
1942 phi29: 1.07mg/ml, 3.0mg, purity >90%.<br />
<br />
1697 GST-wt3C: 3.03mg/ml, 4.0mg, purity >90%.<br />
<br />
1518 T3 ligase: 2.50mg/ml, 3.5mg, purity >90%.<br />
[[Category:My ELN]]</div>
Crm