Crm: Created page with "Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column. Elute 483 Tth Polymerase with Buffer B 06/05/2017..."

2017-06-05T20:45:17Z

Created page with "Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column. Elute 483 Tth Polymerase with Buffer B 06/05/2017..."

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Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column.

Elute 483 Tth Polymerase with Buffer B 06/05/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.03 
| -0.02 
| -0.08 
| -0.12 
| -0.13 
| (1~5) 
|-
| -0.11 
| -0.05 
| 0.13 
| 0.50 
| 1.18 
| (6~10) 
|-
| 2.92 
| 1.33 
| 0.51 
| 0.27 
| 0.17 
| (11~15) 
|-
| 0.17 
| 0.08 
| 0.04 
| 0.04 
| 0.03 
| (16~20) 
|}

Culture 1464 T7 DNA Ligase 8L, induce with IPTG.

Culture 1946 TdT bovine 200ml.

Prepare LB/Amp agar.

Plasmid transformation of ligation sample 1 and 2.

Mini prep of 1420

Prepare LB media.

Deal with order 4873.

[[Category:My ELN]]

MS 6 5 2017 - Revision history

Crm: Created page with "Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column. Elute 483 Tth Polymerase with Buffer B 06/05/2017..."