Crm at 13:54, 9 June 2017

2017-06-09T13:54:42Z

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Spin down 1464 T7 DNA Ligase, homogenize, centrifugate and load onto Ni column.

Culture 1946 TdT bovine 8L, induce with IPTG.

Culture 1497 sso7d-ToughTaq 200ml.

Elute QHP column (load with 483 Tth Polymerase)

[[File:125-483 Tth Polymerase QHP 6 6 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1782 Tth ligase and 483 Tth polymerase 06/06

{| style="width:500px" cellspacing="1" cellpadding="0" border="1" width="500"
|-
| 
| colspan="3" |
1782 Tth ligase 79.1

|
| colspan="8" |
483 Tth polymerase 96.1

|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
11

|
12

|
16

|
MW

|
10

|
12

|
14

|
15

|
16

|
18

|
19

|
20

|-
|
uL

|
0.6

|
0.2

|
2.7

|
10

|
2.4

|
1.6

|
2.0

|
2.2

|
1.6

|
2.1

|
3.2

|
6.7

|-
|
ddH2O

|
15

|
15

|
10

|
\

|
10

|
15

|
10

|
10

|
15

|
10

|
10

|
10

|}

[[File:Protein Expression SDS-PAGE 124-1782 Tth Ligase and 125-483 Tth Polymerase QHP 6 6 2017.jpg|RTENOTITLE]]

Dialyse sample 11 and 12 of 1782 Tth ligase, 19 and 20 of 483 Tth polymerase.

1782 Tth ligase totally have 10ml, 10.64mg/ml, 106.4mg. 483 Tth polymerase totally have 13ml, 1.11mg/ml. 14.43mg. Store at -80oC.
[[Category:My ELN]]

MS 6 6 2017 - Revision history

Crm at 13:54, 9 June 2017