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MS 6 7 2017 - Revision history
2026-10-18T06:19:21Z
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Crm at 22:13, 7 June 2017
2017-06-07T22:13:09Z
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<p><b>New page</b></p><div>Culture 1498 Taq DNA Ligase 200ml.<br />
<br />
Culture 1497 sso7d-ToughTaq 8L, induce with IPTG.<br />
<br />
Spin down 1946 TdT bovine.<br />
<br />
Prepare LB media 20L.<br />
<br />
Prepare LB/K agar and LB/K/C agar.<br />
<br />
Plasmid transformation. 1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase.<br />
<br />
Wash Ni column (load with 1464 T7 DNA Ligase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column.<br />
<br />
Elute 1464 T7 DNA Ligase with Buffer B 06/07/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| -0.19<br/><br />
| -0.21<br/><br />
| -0.02<br/><br />
| 1.36<br/><br />
| 2.34<br/><br />
| (1~5)<br/><br />
|-<br />
| 7.36<br/><br />
| 3.66<br/><br />
| 1.11<br/><br />
| 0.65<br/><br />
| 0.34<br/><br />
| (6~10)<br/><br />
|-<br />
| 0.15<br/><br />
| 0.09<br/><br />
| 0.06<br/><br />
| 0.03<br/><br />
| 0.01<br/><br />
| (11~15)<br/><br />
|}<br />
<br />
Collect 4~11.<br />
<br />
1708 Cre Recombinase 1:1 dilute with SP Dilution Buffer. SP FF column wash with SP Elution Buffer and SP Wash Buffer, then load Cre onto SP FF column.<br />
[[Category:My ELN]]</div>
Crm